上皮间质转化(EMT)是肿瘤侵袭转移级联过程的关键步骤，长链非编码RNA（lncRNA）参与了EMT进程。最近研究表明，转化生长因子激活的RNA(lncRNA-ATB)通过竞争性结合miR-200s和IL-11参与EMT形成，但是其在TGF-B诱导的胃癌EMT中的作用尚不完全清楚。前期研究表明消痰散结方能延长胃癌患者生存期，减少原位移植胃癌裸鼠血清IL-8、TGF-β炎症相关因子的浓度、减少FAP蛋白表达的作用，可能是其抑制胃癌侵袭转移的机制之一，因此我们推测消痰散结方可能通过调控lncRNA-ATB抑制胃癌EMT，以达到抑制侵袭转移之目的。本项目通过构建lncRNA-ATB过表达/干扰载体，采用western blot、qRT-PCR等方法，研究lncRNA-ATB参与胃癌EMT过程及调控机制，以及消痰散结方对其干预机制，为抑制胃癌转移提供理论支持，为中西医结合胃癌治疗提供科学依据。

The epithelial-mesenchymal transition(EMT) play a critical role in invasion-metastasis cascade of tumor, long noncoding RNA(lncRNA) has been reported to mediate the EMT in tumor progression. Recent study found that the lncRNA activated by TGF-β(lncRNA-ATB) induced EMT and invasion by competitively binding the miR-200 family and IL-11 mRNA in hepatocellular carcinoma and breast cancer, but its mechanism in gastric cancer(GC) is not fully understood. According to our previous research, Xiaotan Sanjie Formula(XTSJF) could prolong the survival time in GC patients, decrease the concentration of TGF-β and IL-8, inhibit fibroblast activation protein(FAP) expression in experiment study. Accordingly, we presume that XTSJF might inhibit EMT through mediate the lncRNA-ATB in GC metastasis. In present study, we intend to explore the mechanism of the lncRNA-ATB mediate EMT in GC lines, and XTSJF whether or not interfere lncRNA-ATB exprssion to inhabit EMT in GC in vitro and in vivo used western blot、quantitative reverse transcriptase polymerase chain reaction(qRT-PCR) methods. This will provide the experiment basis for the early recognition EMT, and give new insight toward therapeutic intervention against of GC, and provide scientific basis for the TCM and Chinese herbal in gastric cancer treatment.