真菌通过经历不同形态转化以及产生不同类型的次生代谢产物来应对环境条件。捕食线虫真菌 （nematode-trapping fungi）是通过长期进化适应形成的丰富多彩、形态多姿、功能多样的一类特殊的生态菌物类群。这类真菌对调节土壤中线虫的种群动态起到重要作用，也是生物防治线虫危害的主要途径。前期研究发现捕食线虫真菌代表菌株少孢节丛孢能够产生结构新奇的特有的PKS/TPS杂合生源次生代谢产物，研究表明该类不仅具有重要的化学分类学意义且是调控真菌形态，参与真菌繁殖、防御和捕食过程中的一类新型信号小分子。本项目拟开展这些特色PKS/TPS杂合生源类活性小分子化合物的生物合成途径研究，鉴定其生物合成的关键和相关基因，验证基因功能，对阐明新型微生物通讯系统的化学信号构成及传导途径提供科学依据，对揭示捕食线虫真菌的防御和捕食机制提供参考价值。

Fungi produce a diverse array of secondary metabolites and undergo morphological transitions in response to environmental conditions.Nematode-trapping fungi account for a large proportion of predators of nematode in nature, and develop into a variety of a special class of morphological fungi on long-term adaptation and evolution. They regulate soil nematode population dynamics and play an important role in biological control of nematodes. A unique group of oligosporol metabolites of mixed biosynthetic origins have been characterized from Anthrobotrys oligospora.The previous study indicated that these taxonomic group of chemical signals displayed significant autoregulatory effects on the morphology of A. oligospora, which correlate with fungal reproductive,defensive and predatory capabilities, respectively. The project intends to carry out investigation on the biosynthetic pathway of these characteristics PKS/TPS hybrid ingredients, identify the key and related biosynthesis genes, and validate the gene functions. This will help give an insight into of new microbial communication systems and the chemical composition the signal transduction pathway and provide scientific basis for revealing defense and predatory mechanisms of nematode-trapping fungi.