中文摘要
随着人们对绿色农作物需求的大量增加。当前急需培育养分高效吸收的高产作物品种,以减少化肥的施用量。而良好的根构型是养分水分高效吸收的基础。改变根构型以提高养分水分吸收效率将是一个不错的策略。因此,阐明根构型调控的分子机制将具有重要的理论和应用意义。前期本实验室曾筛选得到根向地性减弱的突变体Osgl1(Oryza sativa gravitropic less1),该突变体地上部和野生型没显著差异,在田间表现出更大的根系分布面积,在半施肥的土壤中表现为比野生型显著提高产量。表明该基因具有良好的生产应用潜力。本项目将在克隆根构型调控基因OsGL1基础上进一步阐明该基因的分子生化功能,通过酵母双杂交和抑制子突变体筛选等方法阐明该基因的下游靶基因以及他们参与根向地性及根构型的分子调控机制。以便为养分高效吸收育种提供有用的基因和遗传资源。
英文摘要
With the increasement of our requirement for green crops, it’s emergent to breed high-efficient nutrient uptake high yield crops to lower down the amount of fertilizer application. As elite root architecture is the basis of high nutrient efficiency. It would be a nice strategy to improve root architecture for better nutrient uptake efficiency. Therefore, it will have important theoretical and practical significance to clarify the molecular mechanisms of the regulation of root architecture. We have isolated a mutant years ago, which showed less root gravitropic response with normal shoot growth. It was named Osgl1(Oryza sativa gravitropic less1) according to the phenotype. Osgl1 showed larger root distribution area compared with wild type. It showed significant higher yield than the wild type in the field with half fertilizer application. This indicates that it’s a useful gene for agriculture practice. On the basis of gene cloning, this project will further study the molecular and biochemistry function of OsGL1. To identify its target protein by yeast two hybridization or through screening of suppressor mutant. And to clarify its function and the molecular mechanism of root gravitropic response and root architecture in order to provide useful gene and genetic material for breeding practice.
