腰椎间盘突出症（LIDH）手术治疗风险较大，西药治疗有毒副作用，寻求一种安全、有效、简便的中医治疗方法尤为迫切。本课题组前期临床研究证实：刮痧治疗LIDH临床疗效显著，但机制不明。基础研究证实：刮痧具有抗炎、提高免疫力、神经调节功能等效应，但研究有待深入。而LIDH病变过程涉及特异性蛋白质参与的复杂过程。由此可见，蛋白组学可从整体、系统的角度探讨刮痧治疗LIDH的效应机制。本项目旨在既往研究基础上，采用LIDH大鼠模型，用特定的刮痧方法进行干预，深入探讨刮痧治疗LIDH神经调节功能、抗炎、提高免疫力等作用效应；利用蛋白质组学双向凝胶电泳结合串联质谱技术以及iTRAQ标记的液质联用技术，观察刮痧干预LIDH差异蛋白质的动态变化，探讨刮痧治疗LIDH密切相关的网络途径和可能作用靶点，寻找与LIDH相关的标志性内容物，为揭示刮痧治疗效应机制提供客观依据，为临床合理应用刮痧法治疗LIDH提供依据。

Lumbar intervertebral disc herniation (LIDH) is a common and frequently-occurring disease, for which, scraping therapy has obvious effects. However, its mechanism has still been unclear yet. The previous studies confirmed that scraping therapy has the effects of anti-inflammation, immunity improvement, and nerve function regulation. However, these relevant researches need to be furthered. The pathological changes of LIDH is a complex process involving the participation of specific proteins. While proteomics can integrally and systematically explore the mechanism of scraping treating LIDH. Based on the previous studies, this study will make use of the rats with LIDH as models, compare the local tissue's pathological changes and relevant biological indicators among sham operation group, model group, and scraping group and in the different treatment courses in scraping group, in order to deeply explore and discuss the nerve function regulation, anti-inflammation and immunity improvement of scraping. And then observe the dynamic changes of all proteins in the serum and vertebra disc of rats with LIDH after scraping with the methods of proteomics of two-dimensional gel electrophoresis combined with tandem mass spectrometry and liquid chromatography-mass spectrometry using iTRAQ labeling technique, thus in order to explore the network channels and possible target which is closely related with scraping therapy treating LIDH, to identify the material foundation of scraping effects, and further provide the objective experimental evidence for the mechanism of scraping therapy as well as clinical guidance for LIDH treated by scraping therapy.