子宫内膜种植窗是影响体外受精-胚胎移植妊娠率的主要因素，HOXA10是雌孕激素作用于子宫内膜的重要介导者，对种植窗期进行精密的时空调节，前一国家自然科学基金发现HCG注射日介入电针可改善胚胎着床，预实验表明超排卵改变了HOXA10的表达，电针对其有调节作用，据此提出“电针通过调控HOXA10的表达时相来调节种植窗期以提高妊娠率”的假说。采用超排卵小鼠，运用宫腔内基因转染上调和下调HOXA10的表达，应用FQ-PCR、Western blot等连续观察HOXA10表达，扫描电镜观察胞饮突，阐明体外受精-胚胎移植妊娠率低是因HOXA10表达时相前移，种植窗提前开放和关闭所致；HCG注射日行电针治疗，从HOXA10表达时相介导的种植窗期入手，研究电针提高妊娠率的作用机制。研究成果有望为探索体外受精-胚胎移植和不孕症的治疗提供新的研究思路和理论支撑，也可为该类疾病的药物或物理治疗提供潜在治疗靶点。

Endometrial implantation phase restricts the clinical pregnacy rate in vitro fertilization and embryo transfer (IVF-ET ). HOXA10 is important during estrogen and progesterone functioning in the endometrium and accurately regulates temporal and spatial of endometrial implantation phase. We have found electro-acupuncture with HCG injection may improve embryo implantation in the previous experiments. Moreover, we found ovarian hyperstimulation changed the expression of HOXA10, and could be regulated by electro-acupuncture. So we hypothesize that electro-acupuncture improve endometrial implantation phase through regulating the expression phase of HOXA10, so as to improve the pregnancy rate”. We use controlled ovarian hyperstimulation preparation of mice with expression of HOXA10 knocked-up and knocked-down through endometrial gene transfection, observe the expression phase of HOXA10 with real-time fluorescence quantitative PCR, Western blot and so on, watch the change of pinopodes by the scanning electron microscope, to clarify that IVF-ET pregnancy low rate is due to the expression of HOXA10 phrase forward and planting window in advance opening and closing; treat with electro-acupuncture on the day of HCG injection, to explore the mechanism of electro-acupuncture increasing pregnancy rate based on the endometrium implanation window adjusted by HOXA10. The results is expected to give a new idea and theory support to IVF-ET and the treatment of infertility, but also can provide the possible therapeutic target for drugs and physical stimulation on such disease.