近红外光介导的光热治疗是一种新兴的微创肿瘤治疗技术，然而单一的光热治疗无法完全去除肿瘤细胞，并且常用的无机光热治疗剂普遍存在携载药物困难、靶向性差等缺陷。因此，本项目拟制备一种多功能CuS纳米摇铃（Nanorattle）光热治疗剂，其内核为磁性Fe3O4纳米粒子、保护层为生物相容性良好的明胶蛋白,利用明胶蛋白对温度和酶敏感的特性将化疗药物装载到CuS纳米摇铃的空腔中，实现集肿瘤磁靶向、磁共振成像诊断、肿瘤部位光可控型及酶响应型药物释放以及光热治疗与化疗联合作用于一体。该多功能CuS纳米摇铃光热治疗剂的成功研制不仅能克服单一光热治疗的局限性，解决无机光热治疗剂载药困难等问题，同时能够提高癌症治疗的靶向性，为开发新型光热治疗剂及癌症的有效诊断和治疗奠定理论和技术基础。

Photothermal therapy (PTT) mediated by near-infrared light irradiation has gained popularity as a promising minimally invasive technique for cancer therapy. However, it is difficult to completely eradicate tumor cells by PTT. In addition, due to the lack of functional groups, it is not easy to carry drugs as well as targeting molecules for inorganic PTT agents. In this project we try to develop multifunctional gelatin stabilized CuS nanorattles with superparamagnetic Fe3O4 nanoparticles as their cores and biocompatible gelatin macromolecules as their protectants. Due to the thermo-sensitive and enzyme-sensitive properties of gelatin macromolecules, chemotherapeutic drug is loaded into the hollow CuS nanorattles, realizing tumor magnetic targeting, magnetic resonance imaging for cancer diagnosis, tumor-site specific enzyme and light responsive drug release and combined therapy. The successful development of multifunctional CuS nanorattles PTT agents shows many advantages including overcoming the limitation of single PTT treatment, solving the difficulties of loading drugs into inorganic PTT agents, improving the targeting capability and laying the theoretical and technical foundation for developing novel PTT agents and effective cancer diagnosis and therapy.