中药方剂具有系统性及整体性的特点，现有的经典研究手段很难在分子层面全面阐释中药方剂这一特点。酿酒酵母被广泛用作系统生物学研究的模型生物，拥有完备的细胞生物学研究基础，具备高通量高内涵的研究手段，是目前最完备全面的生物系统模型，本项目拟建立基于酵母体系的中药方剂系统生物学研究关键技术平台，整合化学基因组学、蛋白质组学、转录组学、代谢组学、化学信息学及生物信息学等技术，研究中药方剂多成分、多途径的整体作用机制。同时将该研究平台应用于临床常用中药麝香保心丸的研究，以阐释其治疗冠心病、心绞痛的分子作用机制，为从整体上阐释中药方剂的分子机制提供示范。研究成果将在国际主流刊物上发表高水平研究论文10篇以上，申请专利5项。

Elucidating the underlying mechanism of traditional Chinese medicine via a systems approach has been long perceived yet achieved at molecular level. As the vanguard of systems biology, Saccharomyces cerevisiae has the most comprehensive eukaryotic background, with a plentitude of high throughput technology and the most complete cellular systems models. This project will employ S. cerevisiae as a systems biology platform to harness the knowledge of TCM’s systems pharmacology by integrating quantitative experimentation of chemogenomic, proteomic, transcriptomic and metabolomic with simulations based on multi-level mathematical models. Such global approach will provide novel insights into how the complementary and synergic essence of TCM achieve the therapeutic efficacy via its multiple components interacting with multiple cellular targets in the context of molecular networks. The research results will publish over 10 papers research papers of high level in the international mainstream journals and 5 patents.