近年来，由于ABA受体的发现，揭示了ABA信号通路核心元件组成成员之间的关系。加深对ABA信号通路中关键信号元件ABA受体调控网络的研究，对揭示ABA信号转导的分子机制以及植物抗逆的分子机理都具有重要意义。本实验室前期的研究中，发现一个类受体细胞质蛋白质激酶（RLCK），与ABA受体RCAR3和RCAR11存在相互作用。因此，我们将其命名为CARK1。由于CARK和RCAR家族在拟南芥中分别具有11个和14个成员，本项目拟通过对两个家族相互作用的鉴定、ABA受体的磷酸化以及受体磷酸化后的功能分析、构建CARKs的多重突变体以及在突变体中过表达CARK并分析突变体和转基因植物的表型，明确CARK家族11个成员与RCAR家族14个成员之间的关系。通过对CARK家族和RCAR家族的关系进行深入研究，揭示ABA受体的磷酸化调控过程、调控网络及其对ABA信号传递的影响。

Recently, the finding of ABA receptors reveals the relationship among the key components in ABA signaling pathway. The analysis of the regulatory network of ABA receptors in ABA signaling is very important to understand the molecular action of ABA signaling pathway and molecular mechanism of plant stress resistance. The pre-project work in my laboratory identified an Arabidopsis receptor-like cytoplasmic kinase (RLCK) which interacted with ABA receptors (RCAR3 and RCAR11). Hence, we named it as cytosolic ABA receptor kinase 1 (CARK1). CARK family and RCAR family have 11 and 14 members in Arabidopsis, respectively. In this project, we want to identify the interaction between two families, to analyze the phosphorylation of ABA receptors and the function of phosphorylated ABA receptors, to generate the multiple mutants of CARKs, to express the CARK in the multiple cark mutants and to analyze the phenotype of mutants and transgenic plants. Through the study, we want to reveal the relationship between CARK family and RCAR family and understand the regulatory network of phosphorylation of ABA receptors as well as this effect on ABA signal transduction.