川芎冰片是中医治疗心肌梗死的常用药对，二者配伍包含了“引经”、“行气”、“活血”多种治则，是中医药配伍理论的集中体现。将其与现代科学相结合诠释其内涵，对于科学阐释中医配伍理论，完善现代治疗心肌梗死方案具有重要意义。我们前期研究发现川芎中存在调控ADR、ATP、NF-κB、PPAR等信号通路成分，药理作用与“心血管收缩、能量代谢、免疫调节及糖脂代谢”关系密切；引经药冰片药效成分可以调控P-gp，影响靶器官药物浓度，且与川芎药效成分具有协同作用。本项目拟通过建立心肌梗死模型，开展川芎冰片治疗心肌梗死系统生物学研究；结合活性导向的UPLC-Q/TOF快速筛选平台，辨析川芎冰片药对调控上述通路药效成分；并以化学生物学手段标记关键成分进行活体示踪及靶点垂钓，揭示其作用靶点、通路以及网络的关联；以期阐明川芎冰片药对“引经、行气、活血”协同增效分子机制，为完善现代治疗心肌梗死方案提供理论基础及实验依据。

The combination of Rhizoma Chuanxiong and Borneol is a commonly used couplet-medicine of traditional Chinese medicine in the treatment of myocardial infarction. Their compatibility includes the "guide effect", "promoting Qi", "active blood", which is a concentrated expression of the compatibility of Chinese medicine theory. To combined with modern scientific interpretation to illustrate the meaning of the compatibility of Chinese medicine theory, and improve the treatment methods of myocardial infarction is important. Our previous study found that the signaling pathways of ADR, ATP, NF-κB, PPAR were regulated by the components in Chuanxiong, which leaded to cardiovascular contraction, energy metabolism, lipid metabolism and immune regulation. The "guide effect" of Borneol is related to regulate P-gp, affect the drug concentration in target organs which could show a synergistic effect with Chuanxiong. The project is planned to through the establishment of myocardial infarction model, by system biological studies to investigate the compatibility of Chuanxiong and borneol. By bioactivity-based UPLC-Q / TOF to rapid screen the active components of Chuanxiong and borneol. Associated marks in vivo tracking of critical components and target fishing to reveal the target-pathway network, we try to clarify the synergistic mechanism of Chuanxiong and borneol on the "guide effect", "promoting Qi", "active blood" effect, and by provide a theoretical basis and experimental evidence to improve the treatment of myocardial infarction.