神经再生是缺血性中风神经功能恢复的重要途径，细胞自噬决定神经干细胞生存和功能，机制不明。气虚血瘀为缺血性中风主要中医证型，益气活血为主要治则。补阳还五汤为益气活血代表方，临床和实验研究（包括本课题组前期研究）均证实其恢复神经功能的确切作用，机制不明。中医“气血-肾精-脑髓”与 NSCs密切相关，“气的化生”和细胞自噬密切关联。基于此，本研究提出假说：脑缺血后NSCs自噬活性改变，影响NSCs的生存和功能。补阳还五汤调控NSCs自噬，影响其功能，促神经再生。并从整体、细胞、分子水平，以MCAO大鼠，经自噬抑制剂、诱导剂干预；缺氧复氧的NSCs，SiRNA敲减自噬关键基因；培养的NSCs，经阻断剂阻断两条自噬调控通路等手段处理，经行为学、透射电镜、RT-PCR 、western-blot、免疫荧光双标/三标等检测，观察脑缺血后自噬活性改变对NSC功能的影响及补阳还五汤的作用、机制和靶点。

neurogenesis is an important approach to improve the ischemic stroke neural dysfunction, cell autophagy decided to NSCs survival and function, the mechanism is unclear. TCM holds that qi deficiency blood stasis for ischemic stroke main type, yiqi huoxue as the main therapeutic principle. Buyanghuanwu decoction is the representation of yiqi huoxue. clinical practice and experimental research (including our previous studies) confirmed its exact role of restoring nerve function, but the mechanism need to be researchde. In traditional Chinese medicine, "Qi and blood - kidney essence - brains" is closely related to the NSCs, and "gas" theory closely associated with autophagy. Based on this, this study put forward the hypothesis that endogenous NSCs autophagy activity changes after cerebral ischemia, affect the survival and function of NSCs. Buyanghuanwu decoction regulates the NSCs’ autophagy, influences their proliferation, differentiation and migration, promotes the neurogenesis. And as a whole, cellular and molecular level, we use the MCAO rats, treated by the autophagy inhibitors and inducers; Hypoxia reoxygenation of fetal rat hippocampal NSCs, knocked down or up autophagy key genes by SiRNA; Cultivated NSCs, treated by the blocker to block autophagy respectively control method such as the two signaling pathways, The behavioral science, transmission electron microscopy (sem), RT-PCR Western-blot, immunofluorescence double standard/three standard detection methods, such as, are used to observe how the autophagy activity change after cerebral ischemia and how to influence the proliferation, differentiation and migration of NSCs, finally to discover the function , mechanism and targets of Buyanghuanwu decoction.