十字花科蔬菜富含一类重要的次生代谢物质-硫代葡萄糖苷，其水解产物具有较强的抗虫能力。本项目以硫苷组成和含量差异较大的大白菜和叶用芥菜为材料，研究它们在与专食性、杂食性昆虫互作过程中起到害虫防御作用的关键硫苷组分与含量的变化的异同，并分析害虫取食后生长发育和生理生化代谢的异同；通过转录组测序(RNA-Seq)等方法，对该互作过程中的植物硫苷代谢及调控关键基因在全基因组水平上建立一个基本网络框架，进而深入解析相关抗虫硫苷代谢及调控的分子机理；在此基础上，筛选该互作过程中信号转导和硫苷代谢相关的关键基因，通过qRT-PCR、启动子融合表达等技术分析其时空表达模式，利用过表达和RNA干涉等方法鉴定它们的生物学功能。这些研究将为明确硫苷在十字花科蔬菜与害虫互作过程的作用及害虫取食提高植物抗性的分子机制提供理论支撑，为抗虫十字花科蔬菜新品种培育及蔬菜安全生产提供理论依据和技术支持。

Glucosinolates are an important group of secondary metabolites enriched in Cruciferous vegetables. Some of their hydrolysates are important anti-insect compound. The glucosinolate profiles are significantly different between Chinese cabbage and leaf mustard. Diamondback moth and Beet armyworm have special and omnivorous diets, respectively. So, we planed to find the key glucosinolate component for insect resistance in the interaction process between the two kinds of vegetables and two pests. And the growth, physiological and biochemical metabolism of the pests will also be investigated after feeding. Then, the key genes for glucosinolate metabolism will be screened by RNA-Seq to make a basic network for understanding the molecular mechanism of the regulation and metabolism of glucosinolates during the interactions mentioned above. On this basis, we will screen the key genes on the signal transduction and glucosinolate metabolism during the interactions. The expression pattern of the key genes could be characterized through qRT-PCR, promoter fusion expression, and so on. Their biological functions will be identified by over-expression and RNA interference methods. In a word, these studies will provide a theoretical support for understanding the anti-insect function of glucosinolates in the interaction process between cruciferous vegetables and insects, and the molecular mechanism of the increased insect resistance of plant after insects feeding. It could also lay a theoretical foundation for breeding of new cruciferous vegetables cultivars with enhanced insect-resistance , except for the production of safe vegetables.