云南省每年发生大量家养动物疾病，包括发热、出血性疾病等，导致大量牲畜死亡，给家养动物业带来巨大损失。动物疫病病因不清，且流行时间相对集中在库蠓繁殖期，但其特点与已经发现的蓝舌病毒感染不同，提示这些地区存在其它蠓虫传播的病毒性疾病。为调查未知蠓媒病毒病，本项目组在云南省主要家养动物疫区采集的库蠓中已分离到36株病毒，初步研究结果提示云南省疫区存在1种新的蠓媒病毒并与家畜动物疫病相关。为进一步阐明新分离蠓媒病毒分子特征及其与动物疫病的关系，本申请拟对该新种病毒开展系统研究，包括全基因组序列测定与分析，建立特异性分子生物学和血清学检测方法，继而开展病毒与家养动物疫病关系研究等。此外，还将对其它尚未鉴定病毒分离株开展系统鉴定，明确分类地位和分子遗传特征及其与当地动物疫病关系。本项目对于解释云南省动物疫病病原体，阐明蠓虫传播病毒与我国动物疫病关系，保障我国畜牧业健康发展和畜产品安全具有重要意义。

A large number of domestic animal diseases, including fever, hemorrhagic disease, occurred in Yunnan province every year, whcih caused huge losses to the domestic animal industry. The aetiology of animal disease is still unknown, while the epidemic time of animal diseases concentrated during the breeding season of culicoides, but its characteristics were different from the disease caused by bluetongue virus, a culicoides-borne arbovirus, suggesting that there are likely other culicoides-borne virus diseases besides animal diseases caused by bluetongue virus. To examine the unknown culicoides-borne viruses diseases, 36 strains of culicoides-borne viruses have been isolated from culicoides collected from prevalent area of domestic animal disease in Yunnan Province, the preliminary study on these viruses indicates that there is a novel culicoiodes-borne arboviruses and its relationship with animal diseases in these region. To further elucidate the molecular characteristics and related diseases of these novel viruses, we will definitude phylogenetic and evolutionary characterization on the basis of the genome sequence of novel culicoides-borne viruses. Importantly, the the relationship between the viruses and the local animal disease will be investigated by means of specific molecular biology and serology detection method. In addtional, systematic identification and molecular characteristics of the others virus isolated from culicoides in Yunnan were preformed to determine taxonomic status, molecular genetic characteristics and the relationship between the viruses and the local animal disease. This program fouse on phthogens of animals and the prevention and control of diseases, which are of importance to veterinary service and public health in Yunnan Province.