逆境胁迫是造成小麦减产的主要因素，研究小麦响应逆境的分子机制具有重要意义。申请人在前期工作中从小麦中国春中筛选出13个GAPDH基因，RT-qPCR分析显示其中TaGAPC1基因在逆境胁迫下转录水平显著升高。为探索TaGAPC1基因在小麦逆境响应中的分子机制，本项目拟从以下四方面进行研究：1、构建逆境胁迫下小麦的cDNA文库及EST分析，获得该基因的过表达和沉默的转基因小麦；2、分别通过酵母单杂交和酵母双杂交筛选与其启动子结合的转录因子和与其蛋白结合的互作蛋白；3、检测TaGAPC1基因在小麦各组织中转录水平的表达情况以及在不同时间胁迫下的表达情况，确定其组织表达谱和诱导表达谱；4、结合转基因小麦和正常小麦在逆境胁迫下的转录组测序和代谢组学分析，分析小麦对非生物胁迫的响应机制。通过上述研究，有望揭示TaGAPC1基因响应逆境胁迫的分子机制，从而为抗逆小麦遗传育种提供新的基因资源和理论基础。

Abiotic stress is a complex environmental constraint that limit the wheat production and yield. Thus, understanding the molecular mechanism for abiotic stress response in wheat could provide important information for genetic improvement. In previous work, we identified 13 glyceraldehyde- 3-phosphate dehydrogenase (GAPDH) genes from Triticum aestivum cv. Chinese Spring, and one of the genes TaGAPC1 showed responding to abiotic stresses dramatically. To further explore the details of TaGAPC1 stress-resistance, this project plans to study on four aspects. Firstly, constructing and analysis of a stresses cDNA library, and obtained over-expression and silence of TaGAPC1 transgenic wheat. Secondly, screening transcription factors interacting with promoter of TaGAPC1 by yeast one-hybrid and interacting proteins of TaGAPC1 by yeast two-hybrid. Thirdly, acquire transcriptional profiles of TaGAPC1 in wheat tissues and under abiotic stress by detecting the level of mRNA. Fourth, make clear the way of TaGAPC1 stress-resistance in wheat through comprehensive analysis RNA-sequence and metabolomic datum of transgenic and wild-type wheat under stresses. Through these studies, it is expected to elucidate the molecular mechanism of TaGAPC1 in response to abiotic stresses, thus providing genetic resources and theoretical basis for the stress-resistant wheat heredity and breeding research.