心肌纤维化是心室重构的重要部分，尤其是非心肌梗死区域的弥散性心肌纤维化是心肌梗死后心力衰竭极其重要的病理变化，其定量检测目前仍局限于心肌活检术。因此，非侵入性心脏磁共振（CMR）成像技术对临床早期发现、量化心肌纤维化有极其重要的意义。对比剂后T1 mapping技术可以通过测量心肌细胞外体积（ECV）间接定量心肌纤维化，但其灵敏度有限，且肾功能不全患者禁用。本团队自主研发的非对比剂T1ρ mapping序列基于大分子蛋白与自由水的相互作用，可测量细胞间质蛋白含量。本项目将利用7T 磁共振T1ρ mapping技术测量大鼠心肌梗死后心肌纤维化含量（替代性和弥散性纤维化），计算出基于该序列的纤维化指标（FI），并与ECV做相关性分析，辅以病理学验证，最终明确非对比剂T1ρ mapping技术是否可准确量化心肌梗死后心力衰竭的心肌纤维化。

Myocardial fibrosis is fundamental process in ventricular remoding. Diffuse myocardial fibrosis of non-infarct zone is the extremely important pathologic changes in heart failure patients caused by myocardial infarction, its quantification has been limited by myocardial biopsy yet. It is significant that myocardial fibrosis is quantified by non-invasive cardiac magnetic resonance(CMR) early. Myocardial fibrosis can be quantified by post-cotrast T1 mapping indirectly through extracelluar volume(ECV) measurement; but this technology is confined by relatively low sensitivity, and it is prohibited for patients with renal insufficiency. Based on the interaction between free water and macromolecular protein, non-contrast T1ρ-mapping sequence self-devolopped can be used to quantify extracellular protein. This project will quantify myocardial fibrosis on 7T MR in myocardial infarction models of rats, myocardial fibrosis index(FI) will be calculated and correlation analysis will also be conducted with ECV, all these will be confirmed by histopathology. Finally, the question that whether non-contrast T1ρ-mapping sequence can quantify myocardial fibrosis of heart failure after myocadial infarction will be solved.

心肌纤维化是心室重构的重要部分，尤其是非心肌梗死区域的弥散性心肌纤维化是心肌梗死后心力衰竭极其重要的病理变化，其定量检测目前仍局限于心肌活检术。因此，非侵入性心脏磁共振（CMR）成像技术对临床早期发现、量化心肌纤维化有极其重要的意义。对比剂后T1 mapping技术可以通过测量心肌细胞外体积（ECV）间接定量心肌纤维化，但其灵敏度有限，且肾功能不全患者禁用。本团队自主研发的非对比剂T1ρ mapping序列基于大分子蛋白与自由水的相互作用，可测量细胞间质蛋白含量。本项目成功编写小动物7.0T MRI T1ρmapping序列，并安装于四川大学分子影像研究室实验室7.0T MRI，并成功获得扫描心脏T1ρ mapping图像。优化临床3.0T T1ρmapping序列，分析基于3.0T MRI T1ρmapping序列得出的纤维化指数（mFI）与基于T1 mapping序列得出的细胞外容积分数（ECV）的相关性；mFI与ECV二者相关系数为r=0.603,p<0.05。并结合病理Masson染色进一步证实了纤维化指数评价心肌纤维化的可行性。进行临床型3.0T T1ρ mapping序列专利申请。MRI多功能负荷实验运动装置项目申请。