创伤后促愈抑瘢是再生医学领域研究的热点和难点。胚胎发育环境是胚胎无疤痕愈合的前提。前期研究表明：1. 微粉化复方ANBP 在动物模型中促愈抑瘢疗效明显；2.创伤后微环境变化可诱导已分化成熟的肌成纤维细胞重编程为表皮干细胞。新近研究表明：Ski是与创伤修复相关的基因，具促愈抑瘢双重疗效，在不同时期双向调节Fb的TGF-β／Smad依赖和非依赖性通路。因此，我们提出“微粉化复方ANBP可能通过双向调节TGF-β通路和ECM等途径，形成类似胚胎发育的最适微环境，促进修复基因适度表达，有效诱导创面Fb出现类似于皮肤胚胎发育不同时期重编程现象”的假说。本项目以FSP1-iCreERT2-GFP转基因小鼠为研究对象, 采用内源性示踪技术，证实微粉化复方ANBP干预后形成类似胚胎发育环境的最佳创伤微环境及真皮Fb重编程为表皮细胞，深入阐明其机理，为实现创伤后无疤痕愈合提供有效的临床干预手段和理论依据。

Double efficacy of enhancing wound healing and inhibiting scar formation after wound is hot spot and difficulty in the field of regenerative medicine research. Early research has shown that: 1. The effect of Pulverized ANBP of Traditional Chinese Medicine Compounds to promote wound healing and suppress the scar in animal models is obvious. 2. The microenvironment changes after trauma can be induced differentiated mature myofibroblasts reprogramming of epidermal stem cells. Recent research shows that Ski genes associated with wound healing, to promote the dual effect, bidirectional regulating in different periods of TGF - beta/Smad through the dependent and independent pathway on Fb. Therefore, we put forward hypothesis " Pulverized compound ANBP can repair dermal three-dimensional structure, balance trauma local microenvironment, the normal repair genes, its mechanism through the bidirectional regulation TGF - beta pathways, inducing fibroblast epidermal cells reprogrammed to epidermalcyts to realize ". This project will be by FSP1 - iCreERT2 - GFP transgenic mice as the research object, using the endogenous tracing technology, mimicking skin embryonic development microenvironmental changes, screening the equilibrium status of local microenvironment and the optimal time point of dermal fibroblasts differentiation、transdifferentiation and programming to epidermal cells in the different periods after wound using ANBP effectively, which provides the theoretical basis to achieve scar-free healing after trauma and effective clinical interventions.