先天免疫在鱼类抵御病原侵害中具有重要作用。Prdm1在哺乳动物先天和后天免疫调控中发挥重要作用。Prdm1可能招募Prmt5发挥其转录调控功能。鱼类有两个同源的prdm1，prdm1a和prdm1b。前期研究发现青鳉Prdm1a和Prdm1b都与Prmt5和Mep50有相互作用，Prdm1a与鱼类先天免疫相关。但是，Prdm1和Prmt5及Mep50的结合方式和结合位点未知，Prdm1a和Prdm1b的功能如何分化、如何在免疫调节中发挥作用还不清楚。本项目拟以青鳉为材料，研究Prdm1a、Prdm1b和Prmt5、Mep50的相互作用及其在先天免疫反应途径特别是NF-κB信号途径中的作用机制。本项目的完成将阐明Prdm1a和Prdm1b与Prmt5和Mep50的相互作用，揭示它们在鱼类先天免疫中的调控作用机制，为深入了解鱼类免疫调控机理增加新的资料，也可以作为高等动物相关研究的参考。

Innate immunity is important for fish defending pathogens. Prdm1 as a transcriptional factor plays a major role in innate and postnatal immune response in mammals. Prdm1 may function by recruiting Prmt5. Fish have two homologous prdm1, prdm1a and prdm1b. We found that both Prdm1a and Prdm1b could bind Prmt5 and/or Mep50 of medaka and that Prdm1a was involved in fish innate immunity. However, the binding pattern and the critical binding sites are unknown, and the functional divergence of Prdm1a and Prdm1b and the regulation mechanisms of Prdm1 in fish innate immunity are in dim. We want to study (1) how these proteins binding each other, (2) how these proteins playing their roles in NF-κB pathway of innate immunity by using medaka as a model. The finish of this project will make us understand the interactions of Prdm1a/Prdm1b and Prmt5 and /or Mep50，will uncover the regulation mechanism of these proteins in the innate immunity of fish, will provide new knowledge of immunity regulation in fish, and will also benefit the studies in mammals as a reference.