课题组自2007年始将针灸疗法介入到不孕症及IVF-ET的临床治疗中，积累了一定的前期研究基础。结合国内外研究进展，本研究拟通过随机对照，在综合评价针灸干预接受IVF-ET治疗的子宫内膜低容受性不孕症患者治疗效应基础上，采用高通量基因芯片技术，筛选子宫内膜容受性的针灸作用基因靶点；利用生物信息学方法，分析针灸干预子宫内膜容受窗期差异表达基因的功能及网络；并应用RT-PCR、Western blot和免疫组化技术，对筛选出的具有已知生物学意义的靶点基因mRNA及蛋白表达进行检测，探讨针灸在基因水平发挥整体调节作用的特点，揭示针灸改善子宫内膜容受性可能的分子作用机制，以期为针灸在不孕症和辅助生育技术中的临床应用提供可靠的科学依据，也为进一步研究针灸对不孕症的分子干预机制提供新的研究方向和研究思路。

We have accumulated some earlier research experiences by applying acupuncture into the infertility and IVF-ET's clinic treatment since 2007. Combining with the research progresses overseas and domestic, and with the overall consideration of the effects of treatment in which we have tried to use acupuncture intervening and IVF-ET to treat the infertility with endometrial low receptivity patients unsuccessfully, we have set a randomized controlled trial, and used high-throughput gene chip technology to screen the gene targets under the acupuncture treatment of endometrial receptivity. And we not only use the bioinformatics to analyze acupuncture intervening endometrial implantation window 'functions and interactive networks of the potential biomakers, but also use RT-PCR, Western blot and immunofluorence to test the known target genes' mRNA and protein expression in our screening. we will discuss the characteristics of acupuncture's function in adjusting the entirety at the genetic level and try to reveal the possible molecula mechanism of acupuncture in improving the endometrial receptivity. So that we may provide a reliable scientific basis for clinical application of acupuncture in infertility and assisted reproductive technology, and also a new research direction and research train of thought for further study of acupuncture in infertility molecular intervention mechanism.