在急性缺血性脑卒中（CIS）发生后静脉用溶栓剂阿替普酶（rt-PA）治疗可以改善患者结局。但溶栓剂可增加致命性颅内出血的发生率，且治疗时间窗短，限制了它的使用。研究表明，rt-PA触发LRP1诱导的基底金属蛋白酶-9（MMP-9）的表达上调，进而破坏血脑屏障（BBB）的完整性，为溶栓过程中颅内出血的主要机制。我们的临床研究显示电针在CIS患者超早期介入治疗可改善中风治疗结局。实验研究显示，电针治疗可以下调CIS患者的缺血灶局部的MMP-9水平。本项目以MCAO小鼠模型研究为对象，采用小鼠MRI，免疫胶体金技术，明胶酶谱法等方法，观察研究电针结合溶栓在 CIS急性期介入治疗后，小鼠脑部局部缺血灶的体积，血脑屏障损坏情况及MMP-9蛋白，LRP1蛋白在缺血半球的表达，探讨电针能减轻溶栓治疗时血脑屏脑屏障损伤，减少颅内出血风险，延长溶栓治疗时间窗的机制。

Clinical studies suggested Recombinant tissue plasminogen activator (rt-PA) improve the outcome for ischemic stroke patients at early time intervals after arterial occlusion, but its use is limited to the first hours after stroke onset due to an increased risk of hemorrhagic transformation over time resulting in enhanced brain injury. Detrimental effects of rt-PA are derived from its ability to activate MMP-9 that in turn contributes to the breakdown of BBB. However, there are little effective treatments to inhibitor MMP-9 in clinical practice. Out previous research proved electro-acupuncture could improve treatment outcome at acute period of ischemic stroke. Laboratory results showed MMP-9 levels in the ischemic focus were upgraded by electro-acupuncture. Our project applies MCAO mouse models, mouse MRI, immune colloidal gold technique，and zymography technique to study the volume and the MMP-9 and LRP1 expression in ischemic focus as well as the condition of BBB. Our purpose of this project is to investigate the mechanism of electro-acupuncture involving inhibition of disruption of BBB and protection acute ischemic patients from hemorrhagic transformation when treated with thrombolysis.