AP2/ERF转录因子参与紫杉醇生物合成，能够调控紫杉醇生物合成途径中的4个关键酶基因，但有关其如何调控紫杉醇生物合成的分子机理的深入研究尚未见报道。本课题组前期研究发现4个MeJA处理表达量升高的AP2/ERF转录因子，并已克隆获得2个AP2/ERF基因cDNA全长，两者分别在红豆杉根和茎中表达量较高。本研究拟利用RT-PCR和RACE方法分离克隆2-3个与紫杉醇生物合成相关的红豆杉AP2/ERF基因，通过CHIP-PCR技术确定其调控紫杉醇生物合成的关键酶基因，并分别将AP2/ERF基因在红豆杉细胞系中过量表达和抑制表达，利用转录组学、蛋白质组学和代谢组学技术分析相应转基因细胞系中基因和蛋白的表达差异以及代谢组分的变化，明确AP2/ERF基因调控紫杉醇生物合成途径的关键步骤，全面系统揭示AP2/ERF调控紫杉醇生物合成的作用及其分子机制，最终为缓解紫杉醇供需矛盾提供新的理论基础。

Taxol, also named Paclitaxel, was firstly isolated from the bark of Taxus brevifolia Pacific yew tree as a powerful and complex anticancer drug. Taxol only constitutes 0.01-0.03% of the the dry weight of the Taxus bark, moreover, the Taxus tree grow very slowly. Therefore, a variety of methods are being carried out to produce more taxol to save more people's lives. . Transcription factors involved in terpenoid secondary metabolites, and AP2/ERF transcription factors involved in the regulation of the taxol biosynthesis. However, the further regulating molecular mechanisms have not been reported. The AP2/ERF transcription factors are widely present in the plant, which related a lot of physiological and biochemical reactions, such as disease resistance, stress tolerance, etc. Previous studies indicated that an AP2/ERF gene that isolated from Taxus cuspidata can bind the promoter of four genes which encoded Taxadiene Synthease, Taxane 5α-hydroxylase,Taxane 10β-hydroxylase, and Taxane 13α-hydroxylase of taxol biosynthesis. Besides, we also found the expression of 4 AP2/ERF genes was increased under MeJA induced from the taxus cell RNA-seq data. Among them, 2 AP2/ERF genes were cloned in our lab. According to our results, both of them expressed higher in roots and stems in Taxus marei, respectively. Further research should be carried out to study their regulating role in taxol biosynthesis.. In this study, the role of AP2/ERF transcription factor regulating taxol biosynthesis in taxus will be investigated by molecular biology methods and omics technology. According to the sequence information that obtained from our previous RNA-seq work and GenBank SRA database, 2-3 AP2/ERF will be cloned using RT-PCR and RACE methods. The CHIP-PCR assay will be performed to find the genes that encoding key enzymes of taxol biosynthesis. The over-expression vector and antisense RNA vector will be constructed to transform to taxus cell, respectively. With RNA-seq, itraq and GC-MS methods, the expression patterns of the genes and proteins that related to taxol biosynthesis and the contents of Taxanes will be investigated in the responsive cell lines. Combined all the described results, the molecular role of AP2/ERF that regulate taxol biosynthesis will be concluded. To understand and employ AP2/ERF regulating the taxol biosynthesis is helpful for meeting the taxol supply problem, and the taxus cell lines improved taxol production obviously is also very important for saving more lives of cancer patients.