由于大水面养殖带来水质污染，发展高密度精养是我国水产养殖发展的必由之路，这要求我们培育出耐低氧的鱼类新品种，因此必须阐明鱼类低氧适应的遗传基础与分子机制。鲫鱼不仅是我国水产养殖的主养品种，而且具有最强的耐低氧特性，但是，不同鲫鱼品种或品系间对低氧的耐受能力有很大的差异。本项目选择不同品种或品系鲫鱼为材料，并选择不耐低氧的鲢鱼作为对照，在比较、测定它们对低氧耐受力的基础上，利用转录组学和蛋白组学等技术，并结合生物信息学分析和基因功能数据，构建鲫鱼低氧基因调控网络，鉴定调控网络中的关键节点基因；分析基因遗传变异与鲫鱼低氧耐受力间的相关关系；制备关键节点基因的同源基因敲除的斑马鱼品系，再通过转基因技术制备鲫鱼低氧调控网络关键节点基因的转基因鱼，阐明这些基因的在体生物学功能；利用生物化学、细胞生物学和分子生物学等技术，揭示这些关键节点基因行使功能的分子机制，为耐低氧鱼类新品种培育提供理论支撑。

It is requirement for breeding fish strains or species with higher hypoxia tolerance due to high -density aquaculture in the future of China, thus, we must undersatand the genetic basis and molecular mechanisms of fish in hypoxia adaptation. Carassius auratus not only is a main species for aquaculture in China, but also has the stongest tolerant ability for hypoxia. However, there are some variations in hypoxia tolerance among different strains or sub-species in Carassius auratus. In this study, we propose to take advantage of different strains or sub-species of Carassius auratus, as well as the silver carp, a fish species with weaker hypoxia tolerance, to compare their ability in hypoxia tolerance. Then, we construct the gene network of Carassius auratus in hypoxia-signaling pathway and identify the key genes in this network by transcriptomics, proteomics and bioinformatics. Subsequently, we analysize the relationship between gene mutation and hypoxia tolerance. To illustrate these key gene's function in vivo, we will knockout the homologous gene of these key genes in zabrafish and make transgenic fish lines. In addition, to reveal the molecular mechanisms of these key genes in hypoxia-signaling pathway, we will take advantage of different techniques in biochemistry, cell biology and molecular biology. The achivements of this study will provide theroretical basis for breeding new species with higher hypoxia tolerance.

由于大水面养殖带来水体污染，发展高密度精养是我国水产养殖发展的必由之路，这要求我们培育出耐低氧的鱼类新品种，因此必须阐明鱼类低氧适应的遗传基础与分子机制。鲫鱼不仅是我国水产养殖的主养品种，而且具有最强的耐低氧特性，但是，不同鲫鱼品种或品系间对低氧的耐受能力有很大的差异。本项目选择不同品种或品系鲫鱼为材料，并选择不耐低氧的鲢鱼作为对照，在比较、测定它们对低氧耐受力的基础上，利用转录组学和蛋白组学等技术，并结合生物信息学分析和基因功能数据，构建了鲫鱼低氧基因调控网络，鉴定出调控网络中的关键节点基因；制备了关键节点基因的同源基因敲除的斑马鱼品系，阐明这些基因的在体生物学功能。此外，利用生物化学、细胞生物学和分子生物学等技术，揭示这些关键节点基因行使功能的分子机制。