本项目选择常见的两种淡水贝类- - 河蚬（Corbicula fluminea）和褶纹冠蚌（Cristaria plicata），作为重金属污染指示生物；选择水体中常见的 Cu2+、Cd2+ 和 Pb2+ 三种重金属作为污染重金属。重点研究河蚬和褶纹冠蚌的重金属富集动力学和组织富集规律；通过分子生物学技术克隆河蚬和褶纹冠蚌与重金属相关的标志物基因；利用荧光定量 PCR 技术检测单一和联合重金属胁迫下标志物基因的表达规律；利用生化方法检测单一和联合重金属胁迫下标志物蛋白酶活性或蛋白表达水平变化等内容。通过上述实验，期望研究标志物基因mRNA表达水平与重金属的种类、浓度和胁迫时间的关系，研究标志物蛋白表达水平与重金属的种类、浓度和胁迫时间的关系等，揭示河蚬和褶纹冠蚌对重金属污染的响应机制，并筛选能够敏感、高效和快速反应水体重金属污染的标志物及其检测方法，为水体重金属污染监测提供科学依据。

Two types of common fresh water bivalves, Corbicula fluminea and Cristaria plicata, were selected as the indicator organism of heavy metal pollution, and copper (Cu2+), cadmium (Cd2+) and plumbum (Pb2+) ions were served as pollution heavy metals in this project. The main research contents in this project consist of the kinetic accumulation characters and accumulation rules in different organs under the single and combined heavy metal stress were studied in C. fluminea and C. plicata. The biomarker gene related to heavy metal of C. fluminea and C. plicata were cloned by molecular biologic technique. The expression laws of biomarker genes under the single and combined heavy metal stress were analyzed by real time PCR. Protease activity or the protein expression of biomarker under the single and combined heavy metal stress were detected by biochemical methods. Through these experiments, it expected to study the relationship between biomarker gene mRNA expression level and heavy metal type and concentration and stress time, and study the relationship between the protein expression level of biomarker and heavy metal type and concentration and stress time. The aims of this project revealed the response mechanisms of C. fluminea and C. plicata to heavy metals pollution, and screened the sensitive，high-performance and rapid biomarker to heavy metal pollution, and provide a scientific basis for water-weight metal pollution monitoring.