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外源镁对艾纳香中l-龙脑积累及药材产量影响的协同调控机制研究

外源镁对艾纳香中l-龙脑积累及药材产量影响的协同调控机制研究
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  • 批准号:81403035
  • 批准年度: 2014年
  • 学科分类:中药资源(H2801) |
  • 项目负责人:王丹
  • 负责人职称:副研究员
  • 依托单位:中国热带农业科学院热带作物品种资源研究所
  • 资助金额:23万元
  • 项目类别:青年科学基金项目
  • 研究期限:2015年01月01日 至 2018年12月31日
  • 中文关键词: 外源;艾纳香;龙脑;药材;产量
  • 英文关键词:Blumea balsamifera (L.) DC.;magnesium;l-borneol;cooperative effect;regulation mechanism

项目摘要

中文摘要

长期以来,l-龙脑含量和药材产量低是限制艾纳香产业发展的主要原因。本课题组前期研究已证实,外源镁可以显著提高艾纳香中l-龙脑含量和药材产量,但是其调控机制尚未明确。因此,本项目拟以艾纳香为研究材料,采用水培方式,叶面喷施镁元素及动态监测等手段,一方面,从植物次生代谢途径,探讨外源镁对l-龙脑及其生物合成中的关键酶龙脑焦磷酸合酶活性和前体物质GPP积累的影响,从而调控l-龙脑积累;另一方面,从植物初生代谢途径,分析外源镁对艾纳香内源激素积累的作用,从而影响初生代谢产物总糖、粗蛋白、粗脂肪、粗纤维和灰分积累,进而影响艾纳香药材产量。基于植物的次生代谢是以初生代谢产物为物质基础,通过综合分析,阐释外源镁对艾纳香中l-龙脑积累及药材产量的影响及其协同调控机制。本项目的开展不仅对提高栽培艾纳香药材产量和质量具有重要的理论价值,也为建立和完善艾纳香规范化生产技术体系奠定了较好的实验基础。

英文摘要

For a long time, the Blumea balsamifera cultivation with low content of l-borneol and low yield has become an important factor on limiting its industrial development. The results in our pre-research have confirmed that exogenous magnesium could significantly enhance the content of l-borneol and medicinal material yield, but its regulation mechanism is not yet clear. Therefore, B. balsamifera is cultivated by hydroponics foliage sprayed magnesium with dynamic monitor. One the one hand, base on plant secondary metabolic pathway, the effect of exogenous magnesium on the content of l-borneol, and the activity of key enzyme (bornyl diphosphate synthase) and content of precursor geranyl diphosphate (GPP) in biosynthesis of l-borneol are studied. It can define the regulation of exogenous magnesium on the accumulation of l-borneol. On the other hand, base on plant primary metabolic pathway, the effect of exogenous magnesium on the endogenous hormone is analyzed. The effect of magnesium on contents of primary metabolites (total sugar, crude protein, crude fat, crude fiber and ash) might also be verified and then explicit the influences on medicinal material yields of B. balsamifera. In addition, plant primary metabolites are the substantial bases for plant secondary metabolism. Through comprehensive analysis, the cooperative regulation mechanism of exogenous magnesium on the accumulation of l-borneol and medicinal material yield in B. balsamifera could be elucidated. In short, there is an important theoretical value to increase the quality and yield of B. balsamifera cultivation. At the same time, it will provide a favourable experimental basis to establish and improve the standardized production technology system of B. balsamifera cultivation.

评估说明

    国家自然科学基金项目“外源镁对艾纳香中l-龙脑积累及药材产量影响的协同调控机制研究”发布于爱科学iikx,并永久归类于相关科学基金导航中,仅供广大科研工作者查询、学习、选题参考。国科金是根据国家发展科学技术的方针、政策和规划,以及科学技术发展方向,面向全国资助基础研究和应用研究,发挥着促进我国基础研究源头创新的作用。国科金的真正价值在于它能否为科学进步和社会发展带来积极的影响。

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