研究发现，慢性疲劳综合征的发生与线粒体能量代谢异常密切相关，现代医学中线粒体能量代谢理论与脾脏功能极为相似。从脾论治，健脾调脾是治疗慢性疲劳综合征重要方法之一。针灸治疗慢性疲劳综合征疗效确切，本研究确立从脾论治的思想，针刺足三里调理脾胃、补中益气治疗慢性疲劳综合征大鼠。通过分子生物学技术，探讨针刺足三里通过AMPK/PGC-1α信号通路改善大鼠肌肉组织线粒体氧化应激，AMPK/ULK1信号通路提高大鼠肌肉组织线粒体自噬功能的机制。从AMPK通路调控线粒体自噬功能的角度，部分揭示从脾论治针刺足三里治疗慢性疲劳综合征的作用机制，为中医临床上防治慢性疲劳综合征提供科学的实验依据。

The studies found that the occurrence of chronic fatigue syndrome is closely related to the abnormal mitochondrial energy metabolism. The theory of mitochondrial energy metabolism in modern medicine is very similar to spleen function of Chinese medicine theory. One of the important treatment methods for chronic fatigue syndrome is treatment from the spleen. Acupuncture on chronic fatigue syndrome has a good therapeutic effect. This project studies acupuncture on Zusanli which strengthen the spleen that treat chronic fatigue syndrome rats. This project discusses the mechanism of acupuncture on Zusanli adjusting mitochondrial oxidative stress of rat muscle tissues from AMPK/PGC-1α signaling pathway and improving mitophagy in rat muscle tissue from AMPK/ULK1 signaling pathway by molecular biology technology. The mechanism of acupuncture on Zusanli treating chronic fatigue syndrome is confirmed and generalized by improved mitophagy from AMPK pathway. The result will shed some lights to the treatment of chronic fatigue syndrome with TCM therapy.