为从蛋白质水平解析Pseudopeziza medicaginis(Lib.)Sacc侵染苜蓿叶片的细胞学事件。在充分认识P. medicaginis生物学特性和侵染过程的基础上，综合运用双向电泳技术(2-DE)、质谱技术（MALDI-TOF-TOF/MS）和生理学方法，系统研究P. medicaginis生长发育及侵染苜蓿叶片过程中生理代谢变化和差异表达蛋白变化规律,揭示差异蛋白表达模式，鉴别与生长和致病过程相关的关键差异表达蛋白；分析苜蓿应答P. medicaginis侵染的主要生理学变化，结合生理学指标，分析关键差异蛋白参与寄主组织病理变化的生理学作用；通过生物信息学分析，分析鉴别关键差异（特异）蛋白功能模块或蛋白可能的生物学功能；克隆关键差异蛋白基因，进行生物信息学分析.为揭示P. medicaginis致病的分子机制，探讨P. medicagini成功侵染寄主采取侵染策略提供依据

In order to analysis Pseudopeziza medicaginis(Lib.)Sacc infecting alfalfa this cytological event from protein level, based on the fully understanding of the biological characteristics and infection process of P. medicaginis, physiological metabolism, pathogenic differential proteomics, gene cloning and expression of related proteins genes during P. medicaginis the growth and its infection of alfalfa leaves will be systematically studied by the comprehensive use of two-dimensional electrophoresis (2-DE), mass spectrometry (MALDI-TOF-TOF/MS) and physiological method. Differential protein expression changes will be analyzed, the key differential expression proteins related to pathogenic process will be identified and their expression pattern will be revealed; main physiological changes of alfalfa responses to P. medicaginis infection will be analyzed, combined with the physiological index, physiological functions of key proteins involved in host tissue pathological changes will also be analyzed; function modules or biological functions of the key differential proteins will be identified by bioinformatics analysis; part of differential protein genes related to the growth and pathogenicity will be cloned, bioinformatics analysis will be studied. These results will lay the foundations for revealing the molecular mechanism of P. medicaginis pathogenicity and for investigating the unique infection strategy of P. medicaginis successfully infects the host.