国内花青素相关研究以提取纯化、理化性质、代谢调控和开发利用等居多，而生理生态相关研究较少，从光保护角度研究花青素生物学功能的报道就更少。国际上花青素光保护研究虽是热点，但多数研究都停留在个体或器官水平，很少有报道阐述其分子机制，于是出现各种假说此消彼长的复杂局面。项目立足于广西主要蔗区特定生态环境，在我们近年花青素相关研究的基础上，以清除活性氧和降低自由能这两种根本光保护机制为切入点，以自育及国外引进抗寒能力有显著差异的甘蔗品种为材料，设置大田及室内试验，借助现代质谱、色谱和分子生物学技术，研究花青素低温抗氧化状况及光能利用特性，揭示其光保护实现的关键生理分子机制；研究花青素合成对甘蔗光合特性、色素调配及N素再利用的影响，揭示其调节机制。项目实施为甘蔗低温光保护研究提供新思路，为回答至今困惑人们的"叶片为什么变红"问题提供新资料，同时为花青素生物学功能假说向生理生态理论层面过渡奠定基础。

The researches on the anthocyanins mainly focus on its extraction and purification process, physical and chemical properties, metabolism modulation and development and utilization in our country, and a few studies have concerned on its ecophysiological functions. Up to date, there have been very rare studies that have explored the photoprotective role of anthocyanin in plants in our country. Although the research on the ecophysiological characteristics of anthocyanins is hot outside of our country, most of them stay at the individual or the cell levels and very few studies have developed into cellular and molecular levels, and thus some hypotheses were growth and decline frequently. Based on our previous study on anthocyanins and the actual ecoenvironmental conditions in the main sugarcane growth region in Guangxi, from the point of scavenging of ROS and decreasing excitation pressure, several varieties with different chilling resistant bred by ourselves or introduced from abroad will be used, and the field and laboratory experiments will be carried out in this project. By means of chromatographic and mass spectrometry and molecular biological techniques, the project will study the antioxidant properties and the light use status of anthocyanins in sugarcane leaves under low temperature to explore its major phtoprotective mechanism from the physiological and molecular perspective. Besides, the project will study the influences of anthocyanins on the photosynthetic characteristics, the balance of pigments and resorption of nitrogen in sugarcane leaves under cold environment and will reveal the regulation mechanism of these properties. The implementation of this project will provide new idea for researches on sugarcane photoprotection and provide new data for the question why leaves are sometimes are red that disturbs us for a long time. In addition, the implementation of this project will promote the fundament changes of the hypothesis on the functions of anthocyanins to the level of theory.