抗稻瘟病品种推广栽培2-3年就容易丧失抗性，为水稻育种和生产带来巨大损失，明确稻瘟菌快速克服水稻抗性的机理对水稻育种具有重要的理论指导意义。研究表明转座子的转位造成的突变是稻瘟菌快速克服水稻抗性的主要突变来源，目前对抗性压力下转座子驱动稻瘟菌快速突变的机理尚不清楚。前期工作中，基于稻瘟菌基因组重测序数据，我们发现稻瘟菌中一类非常活跃的转座子MoTE-1在稻瘟菌基因突变中起重要作用，进一步转录组数据分析发现MoTE-1中包含的一个转座酶基因（MoTE-1ase）能响应水稻与稻瘟菌抗性不亲和互作环境而显著上调表达。基于上述工作基础，本项目将结合酵母单、双杂交技术筛选稻瘟菌中与MoTE-1相关的蛋白，并且明确这些蛋白在MoTE-1响应水稻～稻瘟菌不亲和互作过程中的作用，以期揭示MoTE-1响应水稻～稻瘟菌不亲和互作环境而提高其突变稻瘟菌效率的分子机理。预期研究结果将为可持续控制稻瘟病提供新思路。

Rice cultivars typically become ineffective within 2-3 years due to losing of .resistance to the rice blast fungal pathogen Magnaporthe oryzae, causing huge losses for rice production. Dissection of the molecular mechanism underlying M.oryzae rapid genome mutations will provide us new knowledge for rice resistance breeding. Previously published studies indicated that the translocations of the transposons may be one of the major sources for genome variations and rapid adaption to different host and environment conditions, however, the mechanism is still largely unknown. We recently identified an active transposon, named MoTE-1. Genome re-sequencing data indicated that MoTE-1 may play an important role in M. oryzae mutations. The transcriptome analysis showed that the expression of the MoTE-1ase gene is significantly induced in incompatible reaction compared with the compatible reaction. Based on the above results, in this project, we proposed to screen for the MoTE-1 binding and MoTE-1ase interacting proteins using yeast one hybrid and yeast two hybrid systems, respectively, and study their functions associated with the MoTE-1. A better understanding of the mechanism of M.oryzae mutation driven by MoTE-1 from this project will provide new knowledge and strategies for rice blast disease control.