全球气候变暖及环境破坏的加剧所导致的干旱和土地盐碱化已成为限制水稻产量的主要因素。发掘新的优质耐旱、耐盐遗传资源对开展水稻抗逆分子育种具有重要意义。osa-miR2105是从水稻中分离得到的一个特异microRNA。我们已有的研究显示osa-miR2105通过调控OsbZIP86 mRNA的表达在水稻ABA信号、干旱和盐胁迫响应中起着重要作用。本项目拟采用分子生物学、基因组学等技术研究：(1) osa-miR2105自身调控模式；(2) osa-miR2105调控靶基因OsbZIP86的规律和模式；(3) osa-miR2105/OsbZIP86互作在ABA信号、干旱和盐胁迫响应中的分子调控网络；最终评价osa-miR2105/OsbZIP86互作在水稻抗逆分子育种中的应用价值。本研究将丰富miRNA参与的水稻耐旱和耐盐分子机制理论研究，并为水稻耐旱和耐盐分子育种提供更多的遗传基因资源。

Rice is one of the most important staple crops in the world. Drought and salinization of land caused by climate change, global warming and environmental destruction are the most important factors affecting rice productivity and quality. Discovery of new genetic resources affecting drought and salt tolerance are increasingly important in rice genetic improvement through next generation breeding programs...osa-miR2105 is one non-conserved miRNA that first isolated from rice seed by Xue et al (2009). Our study has shown that by regulating the expression of OsbZIP86, osa-miR2105 affects ABA signaling, drought and salt tolerance in rice. To our knowledge, a systematic functional study of osa-miR2105 and its target gene OsbZIP86 in rice has not been reported yet...Based on the preliminary results, our proposal aims to study: (1) Self regulation model of osa-miR2105; (2) Regulation model of osa-miR2105-target OsbZIP86 interaction; (3) Molecular networks of osa-miR2105/OsbZIP86 involving ABA signaling in drought and salt tolerance of rice. Furthermore, we will evaluate the application of osa-miR2105/OsbZIP86 interaction in improvement of drought and salt tolerance in rice molecular breeding...This project will enrich the theoretical research of rice drought and salt resistance and will provide new genetic resources for improvement of rice drought and salt tolerance by molecular breeding.