MYB转录因子在植物类黄酮次生代谢的调控中起着重要作用。本项目在前期从梨基因组中筛选出R2R3-MYB转录因子家族与梨果实类黄酮合成模式研究的基础上，以类黄酮合成模式具有典型差异的4个梨品种果实为材料，首先研究相关MYB基因的结构域特征，分析其靶基因与调控的类黄酮支路代谢；然后通过相关MYB基因的表达模式与类黄酮合成模式的相关性分析，筛选参与调控类黄酮合成的候选MYB基因；进一步基于酵母单杂、免疫共沉淀等实验手段验证这些转录因子的靶基因，探明梨果实中具有不同结构域特征的MYB转录因子选择性改变若干靶基因转录活性从而调控产物合成的类黄酮合成调控网络；最后利用VIGS技术与瞬时表达技术在梨果实上加以验证。解析梨果实类黄酮代谢中转录因子-结构基因-代谢产物的三级调控网络，为梨育种和果实营养品质调控研究以及植物类黄酮生物合成与调控机理的深入研究提供理论支撑。

MYB transcription factor plays an important role in regulating plant flavonoid secondary metabolism. The pre-project has screened all R2R3-MYB transcription factors from the pear genome and clarified the flavonoid biosynthesis pattern in pear fruits. In the present study, four pear varieties fruits with different flavonoid synthesis mode were used as materials. The first core step in this project is to analyze the sequence features of those MYB genes with possibility in regulating flavonoid biosynthesis and to forecast their target genes and their specific regulated flavonoid metabolism branches. Then through the correlation analysis of the related MYB gene expression patterns and flavonoids synthesis model, the candidate MYB genes involved in the regulation of flavonoid biosynthesis in pear fruits were screened. Furthermore, to verify the mechanism of flavonoid biosynthesis regulatory that MYB transcription factors with specific motifs could selective alter the transcriptional activities of specific flavonoid structural genes and change the quantity of products, the target genes will be tested by yeast one-hybrid experiment and chromatin immunoprecipitation (ChIP) experiment. As the last step of this project, this regulatory network will be finally proved by transient system and VIGS (virus induced gene silencing) system on pear fruits. Parsing the three-tier (Transcription factor-Structural gene-Metabolite) regulatory network of flavonoid biosynthesis in pear fruits could offer theoretical foundation for pear breeding, fruit nutritional quality regulation ,and plant flavonoids biosynthesis and regulation mechanism.