滞育强度分化是滞育昆虫分散风险的生活史策略，由物种的遗传多样性和滞育环境调控，但环境因子对滞育强度调控的生理机制鲜为人知。本项研究以柑橘重大害虫柑桔大实蝇为研究对象，以能量储备与代谢为切入点，探讨环境因子对越冬蛹自然死亡和历期分化的调控效应。首先以落果幼虫为材料，明确越冬前温度和食物供给对滞育蛹能量储备的影响，从而构建4-6个能量储备差异明显的人工种群；然后以人工种群为材料，分别考察滞育前能量储备差异、滞育持续期温度和滞育初期高-低温刺激对蛹滞育强度的影响，并定期监测滞育蛹的呼吸代谢率与能量物存量，明确各人工种群的呼吸代谢特征、滞育强度及其变异系数，揭示柑桔大实蝇越冬蛹滞育强度分化的调控因子及其效应，并阐明其滞育维持与解除的能量代谢机制。研究结果有助于完善昆虫滞育的风险分散策略和能量分配机制，促进柑桔大实蝇测报体系的建立。

Differentiation of diapause intensity is the bet-hedging strategy for diapause insects which is regulated by genetic diversity and diapause conditions, but less is known about the physiological mechanisms of diapause intensity regulated by environmental cues. We use the important citrus insect pest Bactrocera minax (Enderlein) to examine how environmental conditions affect the differentiation on pupal developmental duration and overwintering death through energy storage and metabolism. Firstly, we use the larvae which collected from the dropped citrus fruits to detect the effects of pre-winter temperature and food supply on pupal energy reserves by measuring the carbohydrate and fat contents with pupal weight, and establish 4 to 6 artificial populations with significant diversity of energy storages. Secondly, we use those artificial populations to investigate the effects of pre-diapausing energy reserves, overwintering temperature during diapause maintenance phase and higher-lower temperature stress during diapause initiation phase on the diapause intensity and their variance coefficient, then to explore the respiratory metabolism characteristics by periodly monitoring CO2 production and energy consumption of each artificial population during different diapause phases. Based on the above studies, we hope to conclude the energy metabolic mechanism on the diapause intensity regulation of B. minax pupa that will improve our understanding of the energy allocation theory and the bet-hedging strategy during insect diapause, and to promote establishing of the forcast system of B. minax (Enderlein).