葡萄灰霉病是危害欧洲葡萄生产最大的真菌病害之一，并随着我国葡萄设施栽培的广泛应用而日趋严重。我国葡萄资源蕴藏着丰富的抗病基因，因而发掘山葡萄抗灰霉病基因具有重要的育种价值。项目组前期以高抗（几乎免疫的）灰霉病的山葡萄双优、高感灰霉病的欧洲葡萄红地球及其杂交群体为材料，对接种灰霉菌后的双优、红地球转录组测序，已筛选出大量在灰霉菌（Botryis cinerea）诱导下特异表达和增强表达的基因片段。在此基础上，本项目拟通过基因结构分析与功能预测，结合差异基因在接种灰霉菌后双优、红地球及其抗、感病杂种后代植株间的差异表达分析，发掘葡萄抗灰霉病关键基因；再利用同源克隆或cDNA末端快速扩增（RACE）技术克隆抗灰霉病关键基因全长，并通过遗传转化验证基因功能，进而通过酵母双杂交和染色质免疫共沉淀筛选其互作调控基因，明晰葡萄抗灰霉病调控路径，阐明抗灰霉病机理，为葡萄抗灰霉病育种提供理论依据。

European grapevines (Vitis vinifera) are the most widely planted grapes both for fresh consumption and wine production in the word. However, most grape cultivars of V. vinifera are highly susceptible to a fungal disease, grape grey mould, caused by Botrytis cinerea Pers.. Compared to European grapes, Chinese wild Vitis germplasm are resistant to many fungal pathogens, which make them valuable and promising resources for breeding programs of grey mould resistance breeding. Previously, our group found a Chinese wild Vitis, V. amurensis Rpur cv ‘Shuangyou’ was highly resistant to grey mould and we also have the F1 segregating population of this resistant genotype hybrided with V. vinifera cv ‘Red Globe’, which is highly susceptible to B.cinerea. Firstly, we want to use RNA-Seq technology to identify differentially expressed genes (DEGs) in both resistant grape (‘Shuangyou’) and susceptible grape (‘Red Globe’) challenged by B.cinerea in different time points. We will select DEGs that showing different expression patterns in these two genotypes and then narrow down to those DEGs with functional annotations related to disease resistance. In addition, we will also use resistant F1 population and susceptible F1 population to further testify the association of the grey mould resistance and our resistanct gene candidates. At last, we will use transgenic technology to verify the function of these resistance genes. And other methods, such as yeast two-hybrid system and chromatin immunoprecipitation assay, will be also applied to find other interacting genes. We believe that our identified grey mould resistant genes will facilitate studies on the molecular mechanisms of grey mould resistant and contribute to the grape breeding programs.