能量代谢与种植体周围炎密切相关，以AMPK/SIRT1信号通路最为关键，葛根素作为重要的抗炎中药活性物质，能否通过AMPK/SIRT1通路成为抗种植体周围炎新药是本研究的重点。本研究前期对AMPK/SIRT1通路的相关研究已证明葛根素可减弱牙龈卟啉单胞菌毒素对牙龈成纤维细胞的毒性作用，且明显减弱炎性因子IL-1β和IL-6表达，在常规用药剂量下无毒副作用。同时发现AMPK激活可减弱IL-1β，IL-6表达，提示葛根素防治种植体周围炎与激活AMPK/SIRT1通路相关。本研究拟通过建立种植体周围炎模型，MicroCT监测种植体周围组织及基因定点敲除等技术，系统研究牙龈成纤维细胞AMPK/SIRT1信号通路激活对种植体周围炎的影响，阐明葛根素在防治种植体周围炎中的药效和分子调节机制。本研究为防治种植体周围炎早期病变提出新的作用靶点，为葛根素防治种植体周围炎中医药临床防治提供新的理论和科学依据。

Energe metabolism was closely related with peri-implantitis, and AMPK/SIRT1 was one of most important pathway. So whether puerarin could be a new kind of drug with high effective and low toxicity for anti peri-implantitis through AMPK/SIRT1 pathway is the key point of this study. The previous study did related research about AMPK/SIRT1 pathway, which proved lesion of gingival fibroblast caused by Porphyromonas gingivalis toxin (PgTox) was attenuated by puerarin. And, expression of IL-1β, IL-6 was attenuated, either. Otherwise, there were no toxic and side effect of puerarin with conventional dose. The study as well as indicated that expression of IL-1β, IL-6 was attenuated by AMPK phosphorylation which illustrated peri-implantitis therapy by puerarin was related to AMPK/SIRT1 pathway. In this study, advanced technics like setting up peri-implantitis animal model, Micro CT to monitor peri-implant tissues and specific gene knock out, et al will be used in vivo and in vitro study to test whether AMPK/SIRT1 pathway affects inflammatory factors in gingival fibroblast and illuminate the pesticide effect and biochemistry mechanism of puerarin in peri-implantitis. This study will provide a new basis of pharmacological mechanism of puerarin to treat peri-implantitis, and a new basis of prevention of peri-implantitis by traditional Chinese medicine.