中文摘要
影响油菜籽含油量有很多因素,我们在前期的研究中发现,种子成熟后期的脂肪酸消减现象是影响含油量的重要因素。脂肪酸消减是指含油量达到峰值后,在收获前下降的现象。在一些雨水较多的年份,一些品种由于休眠机制不完善,其种子在母体植株上萌动并发芽。种子在收获前的萌动会严重影响含油量,且对菜籽其它营养品质也有不良影响。在这项研究中,我们旨在探明(1)油菜不同种之间、种内不同品种之间种子休眠性的基因型差异;(2) 休眠性差异与种子成熟后期脂肪酸消减的相关性、对最终含油量的影响程度;(3) 油菜种子成熟阶段与种子萌动阶段基因表达谱的差别、及导致两者差别的关键上游调控因子,及相对应的下游靶基因;(4) 通过修饰特定基因,或者选择特定基因的有利等位变化来促进种子休眠、抑制种子提早萌动与油脂消减的可行性。过去人们提升种子含油量的努力大都放在促进油脂的合成上面,我们从完善休眠机制的角度来抑制油脂降解是一崭新的视角。
英文摘要
There are various factors that affect seed oil formation. Our previous research discovered that the rapeseed oil content declines after reaching a peak point, which we named it as “seed fatty acids reduction”. In some years with too much rainfall before harvest time, seeds of some rapeseed genotype germinate on plant, which causes seed oil content reduction as well as downgrading of other seed qualitative traits. In this study, we aim to investigate (1) the genotypic variation of seed dormancy inter-species and within a species; (2) the relations between degree of seed dormancy and the pre-harvest seed fatty acids decomposition; (3) the differences of transcriptome between developing and germinating seeds, and the key regulators leading to the differences; (4) the feasibilities to promote seed dormancy by manipulating relevant genes and/or selecting allelic variations favorable to seed dormancy completion. Previous researches attempted to promote rapeseed oil content by increasing the amount of fatty acid synthesis; ours differs from those by improving seed dormancy and repressing pre-harvest seed fatty acid decomposition.
