病理性心肌重塑涉及表观遗传修饰和非编码RNA的表达变化。如何克服心脏疾病中的表观遗传修饰扰乱是研究者需要面对的一个科学问题。作为一类新型的非编码小RNA ，piRNA在基因沉默和表观遗传修饰方面具有重要功能。然而在心肌损伤中piRNA的调控功能尚不清楚。在本项目中，申请人通过高通量测序的方法筛选到了一种在心梗组织中高表达的piRNA，初步研究结果表明，这种piRNA靶向作用于Bcl-2启动子的CpG比邻区，过表达的piRNA抑制Bcl-2的转录表达，piRNA抑制剂则促进Bcl-2的表达.由此申请人提出piRNA介导Bcl-2表观遗传重塑进而调控心肌细胞凋亡的假说。本项目拟进一步开展深入研究，在细胞和动物水平上明确piRNA在心肌细胞凋亡中的功能，揭示其表观遗传调控机制，阐明piRNA在心肌细胞命运调控中的作用，为心肌梗死的防治和修复提供一个新的视角。

Myocardial pathological remodeling involves epigenetic modification and non-coding RNA expression changes. How to overcome disorderly epigenetic suppression in heart disease is a problem for researchers. As a novel class of small non-coding RNA, piRNAs have important functions in gene silencing and epigenetic modification. It is unknown whether piRNA also can significantly expressed and involved in the regulation of heart disease.In this project, the applicant of get a piRNA of high expression in myocardial infarction through sequencing method, preliminary results show that this piRNA targeted for the Bcl-2 promoter CpG near area. over expression of piRNA, prevent the transcription of the Bcl-2,however piRNA inhibitors is to promote the expression of Bcl-2. The applicant Put forward the hypothesis that piRNA mediated the Bcl-2 epigenetic reshape and regulate apoptosis of myocardial cell. This project intends to further in-depth research piRNA function in myocardial cell apoptosis, reveals the mechanism of epigenetic regulation in cell and animal level, clarify piRNA role in myocardial cell fate regulation and provide a new perspective.for the prevention and repair of myocardial infarction.