卵巢功能在女性生殖生理中非常重要，卵巢早衰治疗棘手。以化疗源性卵巢早衰雌性大鼠为载体，采用补肾填精代表方左归丸灌胃治疗，以戊酸雌二醇为阳性对照，通过两步酶消化法和免疫磁珠法对卵巢生殖干细胞分离、培养，研究化疗源性卵巢早衰大鼠卵巢生殖干细胞的生物学特性及Notch信号通路的调控机制，重点研究左归丸对卵巢生殖干细胞增殖、分化的影响及对Notch信号通路调控的影响，阐释补肾填精法治疗化疗源性卵巢早衰的机制，对研究卵巢老化机制、探讨卵巢早衰的有效治疗、深化“肾主生殖”的内涵具有重要的意义。

Ovarian function is very important in female reproductive physiology in the treatment of intractable premature ovarian failure. The source of chemotherapy of premature ovarian failure in female rats as the carrier, the representative of Bushentianjing side Zuoguiwan treated with estradiol valerate, as positive control, isolation and culture of stem cells on the ovary through two step enzyme digestion and immunomagnetic beads, chemotherapy derived study of ovarian premature ovarian failure rat germ stem regulation the biological characteristics and mechanism of Notch signaling pathway in cells, focusing on Zuoguiwan stem cell proliferation and differentiation of ovarian germ and influence on the regulation of Notch signaling pathway, the mechanism of interpretation of Bushentianjing treatment of chemotherapy of premature ovarian failure, is of great significance to study the aging mechanism, the effective study of ovarian premature ovarian failure the treatment, deepen the "kidney dominating reproduction" connotation.