线粒体遗传机制研究对于杂种优势利用以及线粒体进化等都具有重要的意义。绝大多数被子植物线粒体为母系遗传，而栽培黄瓜线粒体为父系遗传。我们前期研究已发现黄瓜父系遗传是在生殖细胞中存在抑制核酸酶对线粒体DNA降解的机制，而黄瓜拟核蛋白CsWHY2基因参与了这一过程，防止线粒体DNA被降解。但是CsWHY2调控线粒体父系遗传的具体作用和机理尚不清楚。本项目在申请者前期对线粒体研究建立的黄瓜原生质体瞬时表达体系、拟南芥特异表达RFP/GFP体系的基础上，采用RNA、蛋白定量分析等方法，研究花粉发育过程中CsWHY2在调控生殖细胞线粒体DNA的具体作用；结合转基因技术，分析杂交F1线粒体DNA来源，明确CsWHY2对线粒体遗传的调控。研究结果将探明CsWHY2在黄瓜线粒体父系遗传中的分子调控机理，揭示父系遗传中生殖细胞线粒体DNA的保护机制，对于被子植物非孟德尔遗传的分子调控机理研究具有重要指导意义。

Study of mitochondrial inheritance has great significance for heterosis utilization and mitochondrial evolution. The vast majority of angiosperm shows the maternal inheritance of mitochondria, but cucumber shows the paternal. Our preliminary study results show that the mitochondrial DNA of cucumber generative cells were protected and inhibited the degradation of nuclease is the main mechanism of mitochondrial paternal inheritance. Furthermore, the CsWHY2 is involved in protection of mitochondrial DNA in vitro, but the role and mechanism of CsWHY2 gene regulated the paternal inheritance of mitochondria is not clear. With those progress newly made, the project proposed herein aim at the functional analysis of CsWHY2 by cucumber protoplast transient expression system and specific expression RFP/GFP in Arabidopsis vegetative cells and sperm cells system. And elucidate the patrilineal genetic mechanism by tracing the mitochondrial origin of F1 hybrids and mitochondrial metabolism with transformation technique. Expected results would elucidate the mechanism of CsWHY2 gene regulated paternal transmission of the mitochondria in cucumber, and further reveal the protection mechanism of mitochondrial DNA, which could have the important guiding significance for the study of molecular non-Mendelian genetic mechanism in angiosperm.