本课题拟在“微生物-肠-脑轴”理论指导下，结合临床研究和动物实验，探讨ADHD与免疫的关系及安神定志灵的干预作用。运用代谢组学及分子生物学相关实验方法对粪便、血浆、脑组织等样本进行分析，了解肠道菌群和内源性代谢产物的种类，研究与疾病相关的差异性指标；检测疾病治疗过程中炎症指标的变化，分析其与肠道菌群及内源性代谢物的相关性，初步建立肠道微生物、免疫系统和ADHD之间的联系。同时探索安神定志灵对ADHD儿童肠道菌群、内源性代谢产物、免疫系统的影响及其调节中枢神经免疫的作用机制。最终通过微生物-肠-脑轴初步阐明ADHD与免疫的关系及安神定志灵的干预作用，为ADHD的发病机制研究提供新的思路。

The research project, based on the Brain-Gut-Microbiota Axis theory, supported by clinical research and animal experiments, is to discuss the relationship between ADHD and immune system, and the interventional effect of Anshen Ding Zhi Ling decoction. Using relative experimental approaches in metabonomics and molecular biology, samples of stool, plasma and brain tissue were analyzed. The changes of inflammation markers in therapeutic process were tested to analyze its correlation with intestinal flora and endogenous metabolites, so as to establish the relationship among ADHD, enteric microorganisms, and immune system. This project is also to discuss the effect of Anshen Ding Zhi Ling decoction on children’s intestinal flora, endogenous metabolites and immune system, and its mechanism of regulating immune function in central nervous system. Using the theory, the relationship between ADHD and immunity can be clarified and the interventional effect can be expounded, which may provide a new research orientation for the nosogenesis of ADHD.