目前鱼类毒理学的模式生物以淡水鱼类为主，对海洋鱼类的研究严重不足。海洋青鱂鱼（Oryzias melastigma）对各类污染物反应敏感且对盐度有广泛的适应性，是海洋生态毒理学和研究鱼类盐适应机理的理想模式生物，但目前其基因组结构和盐适应的机制仍不清楚。本项目以海洋青鱂鱼为对象，利用高通量测序技术结合优化的组装算法对其进行基因组测序、组装，并注释其结构和功能。通过淡水鱼类日本青鳉鱼（O. latipes）和海洋青鱂鱼的基因组的对比分析，寻找两者之间差异位点与耐盐性相关的候选基因，再将这些候选基因转入狭盐性的花斑青鱂（O. marmoratus）或斑马鱼中去验证它们在鱼类盐度适应中的作用。海洋青鱂鱼基因组的完成能增强其在海洋毒理学中的应用价值，对研究污染物对海洋生物生长和发育的影响有着重要意义，并将为揭示鱼类对高盐度适应的分子机制和促进盐碱水和海水中的水产养殖提供重要理论基础和科学依据。

Most model species in toxicology are freshwater fishes and studies based on marine fishes fall far behind. Marine medaka (Oryzias melastigma) is sensitive to pollutants and is adapted to a wide range of salinity, therefore it is a very promising model organism in marine toxicology studies. However, information about its genomic structure and molecular mechanisms of salinity tolerance is still elusive. Herein, we propose to sequence, assemble, and structurally and functionally annotate the marine medaka genome. Using genomic information from Japanese medaka (O. latipes) and a more efficient genome assembling protocol, we will be able to greatly reduce the cost and time used in the genome sequencing. Through comparative genomic approaches, we will look for differences in two genomes and find the genes responsible for salinity tolerance. These genes will be further transferred into stenohaline fishes such as O. marmoratus or zebrafish. The new marine medaka genome will enhance its values in the ocean toxicology, help researchers to better study pollution in the marine environment,provide bases to find the mechanisms of salinity tolerance in fish and develop aquaculture industry in salty water and ocean.