密度和行距配置是调控产量和抗倒性形成的重要栽培措施，可在不增加其他投入前提下，通过株型结构的改善，合理利用光能，提高作物产量与抗倒性。本课题组前期研究发现，油菜合理的密度和行距配置，可在提高油菜抗倒性的同时提高产量，协调了倒伏和高产之间的矛盾，但尚缺乏相关机理研究。本研究选择株型结构差异显著的2个油菜品种，设置不同的密度和行距配置试验，通过碳同化物质/酶活检测、蛋白质组和qPCR等方法，探索关键物质含量、酶活性、蛋白表达量和编码基因表达差异。通过本项目的研究，不但可从生理与分子层面系统揭示密度和行距配置协调油菜机收产量和抗倒性的作用机理，还有助于丰富油菜产量和抗倒性形成理论，为选育高产抗倒油菜品种和探索油菜轻简栽培技术提供理论依据。

Plant density and row spacing have played a vital role in regulating yield and lodging resistance among agronomic practices. The appropriate plant density and row spacing resulted in structural changes and increase in radiation use efficiency that optimize both the seed yield and lodging resistance without other inputs. Our early study found that the yield and lodging resistance also could be increased and were in harmony under the optimal plant density in combination with row spacing. But it is lack of related mechanism research. In this study, different plant density and row spacing arrangements with two varities of different structure were setted to invesgate the carbohydrates, enzyme activities, proteomic changes and genes expressions by molecular biology techniques. Through this research, the physiology and molecular mechanism to coordinating yield and lodging resistance in rapeseed will be revealed as affected by the density and the row spacing; and help to enrich the theory for yield and lodging resistance formation. It will also provide the theoretical basis for breeding cultivars with high yield and lodging resistance and exploring simple cultivation techniques in rapeseed production.