S.aureus是最重要的奶牛乳腺炎致病菌，国内外针对S.aureus疫苗的研究做了大量探索，但均未取得满意效果，究其原因，主要是这些疫苗没能诱导机体产生良好的免疫应答特别是乳腺免疫应答。近年来研究发现，黏膜上皮细胞含有大量FcRn，而且当其与抗原结合的IgG Fc片段特异性结合后，可介导胞吞转运作用转运抗原穿过黏膜屏障，刺激免疫细胞产生免疫应答。乳头管和乳池粘膜也有大量FcRn,根据这些研究结果，本研究拟将以S.aureus的荚膜多糖和黏附素结合蛋白为基础构建的多价毒力因子疫苗中构入IgG Fc片段，并利用制备的抗体构建抗原抗体复合物疫苗，通过乳头管、乳腺基底部以及肌肉注射等不同途径对试验大鼠进行免疫，比较构建的不同组成的疫苗和不同免疫途径对大鼠乳腺免疫应答的影响，从而确定乳腺中的FcRn是否可转运抗原及这种转运是否依赖IgG完整性，为研制有效的奶牛S.aureus性乳房炎疫苗打下基础。

Staphylococcus arues is the most significant pathogens.Domestic and foreigen researches indicated the effect of vaccine for S.aureus caused bovine mastitis is unsatisifactory.According to recently researches,there are a lot of FcRn in the mucous epithelium which could specially bind to Fc fragment when it link the antigen.FcRn could mediate transcytosis to transport antigen across mucous to act antigen submission fuction to induce special immunity of mammary gland.There are numerous FcRn in teat duct mucous epithelium and gland cistern mucous epithelium.Consequenty,we will use the vaccine based on capsular polysaccharide and adhesins polybalent virulence factors which bind to IgG Fc fragment and produce antigen-antibody complex vaccine by mammary gland immunity,teat duct injection immunity and intramuscular injection immunity to do the rat immunization and dicuss the effects of the vaccine by different vaccine compositions and different immunization methods,which establishes the effective vaccine of bovine mastitis of S.aureus induced Mastitis in dairy cows.