痛情绪是指伤害性和非伤害性刺激所引发的厌恶、恐惧、焦虑等负性情绪，慢性疼痛患者中痛情绪的发生率约70%。杏仁核被认为是中枢边缘系统中痛情绪调节的关键核团，已知核团内多种因子和受体参与痛情绪的加工、传递和形成；慢性疼痛中痛情绪的发生可能与杏仁核多种功能蛋白变化有关，采取蛋白质组学技术易于整体、客观地寻找其关键蛋白。已发现电针能有效干预痛情绪、调控杏仁核功能，有可能通过杏仁核中的关键蛋白调节痛情绪。本工作拟以弗氏完全佐剂致大鼠炎性痛模型，在观察不同时间大鼠慢性痛和慢性痛伴痛情绪发生的基础上，采用蛋白质组学技术，筛选杏仁核中持续参与痛情绪发生的差异蛋白；对比电针干预痛情绪大鼠的差异蛋白谱，筛选杏仁核中参与电针干预痛情绪发生的差异蛋白。进一步对差异蛋白进行表达和功能验证，明确杏仁核中参与痛情绪发生以及电针干预的潜在蛋白靶点。本研究将更深层次地说明慢性痛中痛情绪发生机制，阐明电针干预痛情绪的新机制。

Pain emotion is a negative emotion such as aversion, fear and anxiety caused by nociceptive and non-nociceptive stimulation, which coud influence the development and outcome. The morbidity of pain emotion in the chronic pain patients is around 70%. Amygdala is an importane nucleus on emotional modulation, and many receptor are invloved in the process, transmit and formation of pain emotion. Proteomics is an ideal method to screen the key protein of amygdala. In the study, proteomics is performed to screen the proteins about pain emotion. In order to select the key proteins of electro-acupuncture (EA), he protein maps of EA and pain emotion is compared. Furthermore, the key protein of amygdala involved in the pain emotion and EA are screened. The expression and function verification are then performed to clarified. This study will define our research direction on the mechanism of EA on pain emotion.