中文摘要
我国已发展为全球主要的红掌生产基地和消费市场,但在生产中普遍遭受细菌性叶疫病的严重威胁。目前红掌抗疫病遗传机制尚无研究,常规抗病育种缺乏成效,主栽品种多表现为抗性不足。本项目利用已鉴定出的广谱抗性和感病红掌种质,进行自交和不完全双列杂交,构建多个抗病分离体,采取多世代联合分析,结合数量性状主基因-多基因遗传的统计方法,解析红掌细菌性疫病抗性的遗传规律;高通量开发出红掌RGAs分子标记,结合已开发的E-STS(包括EST-SSR和EST-SNP)、通用型SRAP等分子标记,使用'Acropolis'×'Alabama'F1群体,构建高密度遗传连锁图谱;筛选共分离或紧密连锁标记,准确定位出红掌抗疫病QTLs位点。研究结果将为解析红掌叶疫病抗性的遗传机制提供重要信息,加速抗疫病及其他性状的分子育种进程;并推动红掌基因组学和分子遗传学等基础领域研究的开展。
英文摘要
Anthurium (Anthurium andraeanum Linden) is a popular ornamental flower, and China has became one of the main producers and markets in the world. But serious devastation caused by bacterial blight (Xanthomonas axonopodis pv. Dieffenbachiae, Xad) was prevalent in the anthurium industry. For the scarcity of effective research on inheritance of resistance and breeding, which is regarded as the most threatening disease for anthurium production. In this study, based on the identified parent varieties with blight resistance and susceptibility, we emploied self amd incomplete diallel crossing design, combine multiple generations segregation analysis method of major gene plus polygenes mixed genetic model, to investigate the genetic mechanism of blight resistance. In addition, we will use throughput methods for mining and screening of RGAs molecular markers, combine with the developed E-STSs (EST-SSRs, SNPs), to construct genetic linkage map to map QTLs for bacterial blight resistance. Results will reveal the genetic mechanism of blight resistance, facilitate the marker-assisted selection in resistance and other target traits breeding in anthurium. Moreover, this study will enable better expanding of basic research of genomics and molecular genetics in anthurium.
