荞麦富含黄酮、抗性淀粉等生物活性成分，是21世纪倍受青睐的健康食材。荞麦胚乳淀粉发育与合成影响荞麦加工品质。因此，研究荞麦胚乳淀粉发育进程与淀粉合成积累规律，对荞麦加工品质提升具有重要的现实意义。本项目以陕甘宁荞麦生产区甜荞和苦荞主栽品种为对象，以不同氮素含量做基肥，采用碘-碘化钾、TTC染色法及扫描电镜等手段，研究胚乳细胞发育过程中淀粉积累特点及淀粉粒超微结构，探究荞麦籽粒胚乳淀粉合成过程中AGPP、SSS、GBSS、DBE和Q酶等关键酶活性变化，揭示荞麦淀粉合成关键酶活性变化规律及其对氮素调控响应机制；研究不同氮素水平下荞麦籽粒淀粉理化性质特点，揭示氮肥对荞麦胚乳淀粉发育的影响机制及其与荞麦淀粉加工品质的关系。研究结果为荞麦优质栽培与加工利用以及氮素高效利用提供理论依据。

Buckwheat is rich in biological active ingredients, such as resistant starch and flavonoids, is the popularity health food in the 21st century. Buckwheat endosperm development and synthetic effect of buckwheat starch processing quality. Therefore, researching buckwheat growth course of endosperm starch and the accumulation of starch synthesis rule, buckwheat processing quality ascension has important practical significance. In this study，the cultivation variety of common and tartary buckwheat as the object, different nitrogen as fertilizer, using iodine, potassium iodide, TTC staining method and scanning electron microscopy (SEM), the endosperm cells develop in the process of starch accumulation characteristics and ultrastructure of the development of starch granule explore buckwheat seed endosperm starch synthesis process of AGPP, SSS, GBSS, DBE and Q enzyme activity of key enzymes in the change, revealing the key enzymes activity change rule of buckwheat starch synthesis and its response to nitrogen regulation mechanism; Study the buckwheat grain starch physical and chemical properties with different nitrogen levels, reveal the influence of nitrogen fertilizer on the mechanism of buckwheat endosperm starch development and its relationship with buckwheat starch processing quality. The results of the study will be provide theoretical basis for cultivation of buckwheat and processing and nitrogen efficient utilization.