乙肝是严重危害人类健康的世界性疾病。紫红獐芽菜具有清肝利胆，清热解毒功效，民间广泛用于治疗病毒性肝炎，但其药效物质基础和作用机制尚不清晰。前期从紫红獐芽菜分离得到的化合物显示了确切的抗HBV活性。申请人提出紫红獐牙菜抗HBV活性物质群通过对代谢网络整体调节发挥其治疗乙肝的假说。拟用“植物化学组学和代谢组学” 相结合的模式，寻找富集各类活性次生代谢产物，通过体内外实验测定其抗HBV活性，建立活性物质群库；融合代谢组学研究方法和技术，系统研究健康模型、雏鸭乙肝模型和经活性物质群治疗后内源性代谢物整体变化；利用模式识别和典型相关性分析，以生理检测指标为连结点，标量化“活性物质群-内源性代谢物”间相关性；建立活性物质群与机体内源性代谢物的相互作用动态关系，结合生物代谢通路分析，从代谢网络整体调节的角度探寻活性物质群抗HBV协同作用机制。这对中药的物质基础研究将起到很好的范例作用.

Hepatitis B is a serious worldwide disease caused by hepatitis B virus (HBV) infection, Swertia punicea Hemsl. is widely used as a folk medicine to treat viral hepatitis. However, its active components and mechanism of action are still not clear. Some ioslated compounds showed significant anti-HBV actives. The applicant proposed a hypothesis that anti-HBV active substance groups of S. punicea treat hepatitis B by adjusting the metabolic network of the whole.Therefore, we plan to combine "Phytochemommics" and "Metabolommics" to explore the anti-HBV active components group in the S. punicea, and further research their action mechanism via the metabolic network integrated regulation. Using the Phytochemommics method to find and enrich the active components and their secondary metabolites, then determine their anti-HBV active by in vitro/ in vivo experiments to establish the active components in S. punicea. Based on the Metabolomics methods and techniques to compare the whole metabolic network differences among healthy model, HBV duck model and the model treated with active components. The pattern recognition and canonical correlation analysis will be combined to establish the dynamic interactions between them, using the physiological determine indexes as linkage to scale the relationship between "active components and metabolites network " and coupling with biological pathways analysis to explore their synergy therapeutic mechanism from metabolites network integrated regulation. The study will play a good role in the study of the active substances of traditional Chinese medicine.