银耳（Tremella fuciformis），又名白木耳，是一种经济价值很高的食药用真菌。自古以来，银耳就是人们餐桌上的一种珍品，同时也是传统中医药中的一种良药。我国是世界上最早认识、利用和人工栽培银耳的国家，目前更是银耳生产、消费和出口的大国。无论在野生状态下还是在人工栽培时，银耳的生长发育都离不开香灰菌（Hypoxylon sp.）。迄今为止，对银耳与香灰菌相互作用的机理仍知之甚少。本项目拟采用RNA-Seq技术，分别对银耳、香灰菌以及银耳与香灰菌共培养混合的转录组进行测序，并利用从头组装和与参考基因组匹配的方法，分别构建银耳与香灰菌相互作用前后的转录组图谱，通过对比从而鉴定在银耳与香灰菌相互作用过程中差异表达的基因；同时利用RNAi技术研究一些关键差异表达基因的功能，探讨银耳与香灰菌相互作用的分子机理，为进一步丰富真菌生长发育的基础理论，指导银耳的生产实践及提高银耳的产量奠定基础。

Tremella fuciformis, also known as white wood-ear, is an edible and medicinal mushroom with high economic value. Since the ancient times, it is a delicious food for Chinese people and also a famous tonic in Chinese traditional medicine. China is not only the oldest country of recognition, utilization and artificial cultivation of T. fuciformis, but now also the biggest country of production, consumption and export of T. fuciformis in the world. T. fuciformis is always associated with Hypoxylon sp. to grow and develop whether in wild state or in artificial cultivation situation. Up to now, the mechanism of interaction between T. fuciformis and Hypoxylon sp. is still not very clear. In this study, the transcriptomes of T. fuciformis, Hapoxylon sp., and the mixed T. fuciformis and Hypoxylon sp. in co-culture will be investigated by RNA-Seq. The transcriptome maps of T. fuciformis and Hapoxylon sp. before and after interaction with each other will be constructed through de novo assembly and mapping to the two reference genomes. Differentially expressed genes will be identified and functions of some critical differentially expressed genes will be studied using RNAi technique. The results will be used to elucidate the molecular mechanism of interaction between T. fuciformis and Hypoxylon sp. This study could not only further enrich the fundamental theory of fungal growth and development, but also guide the cultivation practice and improve the production of T. fuciformis as well.