松树蜂是国际上具有极高风险的林业外来入侵生物，两年前首次发现已入侵并定殖于我国东北地区樟子松林分，急需有效的监测技术。系统明确繁殖行为，是开发监测技术的理论基础，也是入侵生物学核心内容之一；而繁殖行为离不开化学生态调控，化学生态调控研究是开发监测技术的最佳切入点。本项目通过林间调查和室内行为实验，研究松树蜂在樟子松上羽化、求偶、交尾和产卵等繁殖行为；运用昆虫信息素、植物（或共生菌）挥发物取样技术、GC-EAD、GC-MS、风洞和Y型嗅觉仪等一系列信息化合物分析技术、室内电生理实验、行为实验以及林间监测试验，明确松树蜂配偶和寄主定位信息化学活性物质组份与比例，开发松树蜂引诱剂，并应用于林间种群监测；嗅觉机制方面，利用单感受器记录，研究松树蜂触角外周嗅觉神经对信息化学活性物质的感受特点。最终从“昆虫-植物-共生菌”复合系统阐明松树蜂的繁殖行为与化学生态调控机理，为该虫的有效监测提供新方法。

The sirex woodwasp Sirex noctilio has become an important forestry invasive species in a number of countries and has invaded in northeast China on P. sylvestnis var. mongolica Litv. two years ago. It is urgent to improve the monitoring technique of the invasive pest. The study on the reproductive behaviour is crucial part of optimizing monitoring technique, which is also an important content of invasion biology. On the other hand, the reproductive behaviour are closely related to chemical ecology regulation, which also play an important role in exploiting new pest montoring approach. The Circaian rhythm of S. noctilio adult emergence, calling, mating and oviposition behavior on the host plant P. sylvestnis var. mongolica Litv. are studied by field surveillance and labortory observation. Semiochemicals including S. noctilio pheronome, plant and symbiotic fungus volatiles are exacted and collected. They are identified and quantified gas chromatography-mass spectrometry(GC-MS). Attractiveness of these semiochemicals to S. noctilio adults are based on gas chromatography-electroantennogram detection (GC-EAD). Both wind tunnel, Y-tube bioassay and field tests are conducted to measure the attraction of S. noctilio adults to crude extract or synthetic compounds lures under labortory and field conditions. Using single sensilum recording (SSR), we investigate the mechanism of the semiochemicals coding in the peripheral olfactory nervous system of S. noctilio. In total, the studies will observe the invasive behaviour and elucidate chemical ecology medicated mechanism of S. noctilio, and finally provide a new approach to monitor the pest under the field conditions from “insect-plant- symbiotic fungus” complex.