指状青霉（Penicillium digitatum）侵染引起的绿霉病是最严重的柑橘采后病害。前人和前期研究均发现，柑橘油胞主要芳香组分柠檬烯能显著诱导指状青霉孢子萌发，但对应的作用机制尚不明了。本研究拟以指状青霉分生孢子为对象，分析不同时间柠檬烯诱导对孢子萌发的影响；通过气质联用（GC-MS）鉴定和分析不同时间柠檬烯诱导后孢子内物质代谢规律，通过气相色谱（GC）测定菌悬液和孢子内柠檬烯含量变化，明确柠檬烯的作用；通过转录组测序技术（RNA-seq）和同位素相对标记与定量技术（iTRAQ）获得指状青霉孢子对柠檬烯诱导做出应答的转录和蛋白组证据，关联后进行荧光定量PCR验证，揭示柠檬烯参与的代谢过程；旨在探讨柠檬烯诱导指状青霉孢子萌发的作用机理。项目的开展不仅能丰富人们对柑橘采后绿霉病害发病规律的认识，也为今后通过改变柑橘果实芳香物质含量和组成来控制采后绿霉病发生等具有重要的理论和现实意义。

Green mold caused by Penicillium digitatum is one of the most damaging postharvest diseases in citrus . Both previous and our former studies revealed that, as the principal volatile component in citrus oil gland, limonene can remarkably induce the spore germination of P. digitatum, but the underlying mechanism remains undetermined. In the present research, the effects of limonene at different culture time on the spore germination of P. digitatum will be determined. The metabolism of the substances in spores of P. digitatum after the induction of limonene at different time will be identified and analyzed by gas chromatography−mass spectrometry (GC-MS). Meanwhile, the limonene contents in spore suspensions and in spores will be analyzed by gas chromatography (GC), in an effort to clarify the role of limonene. The transcriptome sequencing (RNA-seq) and isobaric tags for relative and absolute quantitation (iTRAQ) techniques will be adapted to reveal the transcriptomic and proteomic profiles when the spore germination of P. digitatum is induced by limonene. The quantitative real-time PCR will be further conducted to confirm the correlation analysis results of transcriptomic and proteomic profiles, thereby to reveal the metabolic pathway limonene involved in. All these are to explore the potential mechanism of limonene-induced spore germination of P. digitatum. Our present research can not only provide a good opportunity for better understanding of pathogenesis of green mold in citrus fruit, but also has important theoretical and practical guidance for controlling the green mold by changing the compositions and contents of volatile components in citrus fruit in the future.