小麦条锈病是我国小麦生产上发生的主要病害，严重威胁小麦生产。V26系列菌系的出现及其快速大面积传播给小麦安全生产带来新的重大威胁。曲白春为西藏地方小麦品种，近年来连续在四川成都种植，经人工接种V26菌系，均表现为全生育期抗病（抗病程度为免疫）。本研究将利用已经构建的2个曲白春遗传群体，综合多种方法绘制该抗病基因的高精度遗传连锁图谱。首先利用小麦660K SNP芯片快速粗定位目标基因（F2小群体）、进一步利用比较基因组和BSR-seq等技术开发新的抗条锈病基因的分子标记（F2大群体），完成该抗病基因的精细定位。并利用上述获得的紧密连锁的分子标记应用于辅助选择，培育新的抗条锈病品种（系）。本项目的完成，将为小麦抗条锈病育种提供新的抗源材料，丰富小麦条锈病抗性基因资源；有助于解决V26菌系迅速蔓延的问题，同时可为下一步图位克隆该基因打下基础。

Wheat stripe rust is one of the most important disease which can serious threat wheat production. The arise and rapidly propagation of new strain V26 of Puccinia strifformis f. sp. tritici brings the great threat to wheat production safety. Qubaichun is a wheat landrace from Tibet. It showed a good resistance to strain V26 for several years by artificial induced inoculation in Chengdu, Sichuan province. In this study, our purpose is to draw the high precision genetic linkage map of Yrqbc by using the two constructed genetic population. First, the rough location of Yrqbc could be quickly find by using wheat 660K SNP chip and the F2 small population. Second, comparative genomics strategy is used to develop the new markers from rice and Brachypodium distachyon. Third, BSA+RAN-seq method could find the differential expressed genes which can be used for developing molecular marker. Intigration these markers could draw the high precision genetic linkage map, which could be used for map-base gene cloning of Yrqbc. Meanwhile, the genetic markers tighly linked could be used for breeding the V26 resistant wheat varieties (lines). This work could provide new material for resistant wheat breeding and enrich the Wheat stripe rust resistance gene resources. Furthermore, the results can resolve the rapidly spread of V26 and used for map-based cloning of Yrqbc gene.