硫化氢（H2S）是一种内源气体信号分子，在调控植物生长发育和对非生物胁迫响应中发挥重要作用，但逆境下H2S的信号转导路径和下游级联反应及H2S与其他信号分子的互作关系尚不清楚。本项目针对日光温室低温逆境经常导致黄瓜光合受抑，生长滞缓、产量降低等问题，拟利用外源H2S与NO、Ca2+、ROS、ABA、IAA等信号分子供体和清除剂，通过生化、药理和分子生物学等技术手段开展研究：1）探讨H2S与NO、Ca2+、ROS信号分子对低温胁迫的响应；（2）揭示H2S与NO、Ca2+、ROS信号分子的交叉互作及调控光合碳同化提高黄瓜耐冷性机理；3）解析H2S与植物激素信号分子（ABA、SA等）对低温的交互应答方式，探索H2S与植物激素相互应答在调控光合碳同化中的信号转导通路。该研究可为深入开展H2S在植物中的生理功能及其信号转导网络研究提供借鉴，对缓解逆境障碍和增强日光温室黄瓜的适应能力具有重要意义。

Hydrogen sulfide （H2S）is a kind of endogenous gas signal molecule. It plays an important role in regulating plant growth and development, as well as in response to abiotic stress. However, but no information have been provided on the signal transduction way, downstream cascade reaction of H2S and the interaction of H2S with other signal molecules under adversity. This work focuse on the problems, such as inhibition of photosynthesis, limitation of growth, decrease in yield etc. of cucumber caused by chilling stress in solar-greenhouse. We will carried out the following researches by the means of biochemistry, pharmacology, and molecular biology, with the donors or scavengers of H2S, NO, Ca2+， ROS, ABA, IAA, etc. 1) We will study on the response of H2S, NO, Ca2+ and ROS to chilling stress; 2) We will reveal the cross and interaction of H2S with NO, Ca2+ and ROS signals, and the regulation mechanism of H2S signal on photosynthetic carbon assimilation in improving the cold tolerance of cucumber. 3) The interactive response way of H2S and phytohormones signal molecules (ABA, SA) to chilling stress will be analysed, and Subsequently, the signal transduction pathway of H2S and phytohormones in photosynthetic carbon assimilation regulation will be explored. This research may provide guidance for study on the physiological roles of H2S in plants and its signal transduction network, and has significance in alleviating the adversity effect by increasing adaptability of cucumber in solar-greenhouse.