针对构建小麦内源激素低浓度检测新技术与新方法的要求，并解决光学表面等离子共振易受环境基质的影响问题，提出基于核酸适配体金纳米颗粒光学局域表面等离子共振光谱小麦内源激素检测新方法，实现小麦内源激素实时、无标记、高灵敏度等特征的光学生物检测技术。本项目研究金纳米颗粒光学局域表面等离子共振光谱机理与内源激素的光谱响应特性，揭示高精度小麦内源激素生物光学信号快速获取机理，同时探索新颖的金纳米复合物颗粒对小麦内源激素快速测定的技术内涵。研究小麦内源激素光学局域表面等离子共振波长峰值求解算法 ，小麦内源激素生物分子识别膜和信号放大机制，小麦内源激素与分子识别膜动力学过程，构建一种光学局域表面等离子共振小麦内源激素分析检测新体系，寻求一种检测限低于1ng/g 小麦内源激素检测方案，开拓光学生物传感技术在内源激素超微量检测中的应用。

A novel method for the detection of endogenous hormones in wheat based on the localized surface plasmon resonance spectroscopy of aptamer-functionalized gold nanoparticles is proposed in order to solve these problems caused by the environmental matrix in the analysis of biological samples at a low concentration when the optical surface plasmon resonance is used. The real-time, label-free detection of endogenous hormones in wheat is developed with a high sensitivity and a low limit of detection.The principle of the localized surface plasmon resonance spectroscopy of aptamer-functionalized gold nanoparticles and nanocomposites, and the characteristics of the response signals from the sample solutions including endogenous hormones will be investigated to reveal the acquisition mechanism of the response signals from the endogenous hormones in wheat at a high precision. Meantime, the inherent property of this rapid detection method of endogenous hormones in wheat based on localized surface plasmon resonance of aptamer-functionalized gold nanoparticles is studied intensively. The algorithm for the solutions of the peak wavelength corresponding to the endogenous hormones in wheat detected by using the optical localized surface plasmon resonance is studied deeply. The biomolecular recognition membrane, the signal amplification mechanism and the biomolecular interaction for the detection of the endogenous hormones in wheat are also studied to construct the endogenous hormone detection system based on the optical localized surface plasmon resonance spectroscopy. The rapid detection scheme of the endogenous hormones at a low limit of detection (less than 1ng/g) obtained from the biological optics technology is established to open a novel way for the detection of the ultramicro plant hormones.