草本植物开花机理研究已取得了重要进展,拟南芥开花调控关键基因如FT、TFL1、SOC1、FLC、SPL、AP1的功能已鉴定，开花主要途径——光周期途径、春化途径和年龄途径等的分子机理已较清楚。而银杏是古老的木本裸子植物、我国特产经济树种，幼年期长达20年才开花，其开花调控分子机制尚不清楚。本项目基于转录组学方法，采用cDNA 文库、EST 技术、PCR和qRT-PCR技术等对银杏开花调控关键基因进行分离、鉴定与表达，阐明银杏开花调控的途径及分子机理：（1）银杏开花调控候选关键基因（GbFT、GbTFL1、GbSOC1、GbFLC、GbSPL、GbAP1）克隆；（2）银杏开花调控候选关键基因转拟南芥和杨树功能鉴定；（3）银杏开花调控途径基因表达验证及分子机理分析。项目完成将首次揭示银杏开花调控的关键基因及分子机理，阐明与草本植物开花调控机理的异同，为银杏分子育种与生产栽培提供理论指导。

Research of Herbaceous plant flowering mechanism has made important progress. The function of the key flowering regulation genes from Arabidopsis thaliana such as FT, TFL1, SOC1, FLC, SPL and AP1 was identified. Molecular mechanism of flowering regulation from photoperiod pathway, vernalization pathway, GA pathway of Arabidopsis thaliana and one new age pathway of the perennial herb, is clear. However, the molecular mechanism of flowering about Ginkgo of juvenile stage for up to 20 years, which is an ancient Gymnosperm and unique economic tree in China, is unclear..In the project we shall clone 6 flowering regulation candidate genes GbFT,GbTFL1,GbSOC1,GbFLC, GbSPL and GbAP1 homologous to Arabidopsis thaliana by using PCR、RT-PCR, based on Ginkgo cDNA and EST library. Furthermore, the function confirmation of these genes will implemented by building sense and anti-sense expression vectors, transferring them to the cells of Arabidopsis thaliana and poplar.The mRNA expression of candidate genes would be determined by using the fluorescence quantitative reverse transcription PCR (qRT-PCR) technology, and the temporal and spatial expression characterization would be analised, in order to identify the key flowering regulation genes,flowering pathway and molecular mechanism in Ginkgo. .We shall firstly identify the key flowering regulation genes in Ginkgo, discover their temporal and spatial expression characterization, and explain molecular mechanism of flowering regulation in Ginkgo, which is of important theoretic and practical value to molecular breeding and flowering regulation in Ginkgo, and provide the basis for studying Gymnosperm flowering molecular mechanism.